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  4. Bovine Cortisol ELISA Kit

Bovine Cortisol ELISA Kit

文献引用 (36) 说明书下载
  • 中文名称：
    牛皮质醇(Cortisol)ELISA试剂盒
  • 货号：
    CSB-E13064B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        COR，全名Corin，是一种跨膜型蛋白，主要在心脏和肾脏中表达，参与心血管调节等生物过程。它作为一种膜结合型酶，可以分解脑钠肽等心血管调节分子，影响血压、血容量和心血管功能。COR在心衰、高血压等心血管疾病的发生和发展中扮演重要角色，成为研究的热点之一。
        华美生物所提供的Bovine Cortisol ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测牛血清、血浆、组织匀浆样本中的COR，其灵敏度为0.049 ng/mL，检测范围为0.049 ng/mL-200 ng/mL。
     
  • 缩写：
    COR
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.049 ng/mL-200 ng/mL
  • 灵敏度：
    0.049 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-99  
    1:2 Average % 96  
    Range % 92-105  
    1:4 Average % 85  
    Range % 81-89  
    1:8 Average % 91  
    Range % 83-98  
  • 回收率：
    The recovery of bovine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 92-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.194 0.184 0.189    
    50 0.328 0.320 0.324    
    12.5 0.507 0.550 0.529    
    3.12 0.900 0.975 0.938    
    0.78 1.314 1.266 1.290    
    0.195 1.653 1.657 1.655    
    0.049 1.915 1.902 1.909    
    0 2.306 2.400 2.353    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

Please provide the following information regarding Bovine Cortisol ELISA Kit (CSB-E13064B):
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What's is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
4. If recombinant protein, is it full length or partial?
5. What is the species of the standard– human or something else?
6. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry.
Cortisol is small molecule and it is the same in all species, so there is no uniprot.
Since for different species, the matrix is different, we have developed kits for different species.
CSB-E13064B
The standard is purified cortisol. This is a competitive kit. The plate is coated with specific antigen. The specific antibody is rabbit polyclonal antibody. The immunogen is modified by special modification.
HRP is conjugated with secondary antibody( goat anti-rabbit antibody).
Pls let me know if you have any further questions. Thank you.
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靶点详情

  • 最新研究进展：
    COR（Cytochrome c oxidase subunit 7B），是呼吸链中的一个亚基，参与线粒体氧化磷酸化过程中的电子传递。最近的研究表明，COR在多种类型的癌症中都具有潜在的作用，如肝癌、胰腺癌、肺癌和乳腺癌等，可以作为潜在的治疗靶点。此外，COR还与神经退行性疾病的发病机制相关，如阿尔茨海默病和帕金森病等。
     

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