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中文名称:鱼类皮质醇(Cortisol)ELISA试剂盒
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货号:CSB-E08487f
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:COR蛋白是存在于人类心肌细胞中的一种蛋白质,它在维护心肌结构完整性和心脏功能方面发挥着重要作用。它能够促进心肌细胞之间的黏附和相互作用,从而形成一个完整的心肌网络。不过,如果COR蛋白的表达量过高,它也会对心肌产生毒性作用,导致心肌病变和心血管疾病的发生。因此,COR蛋白在心血管疾病的诊断和治疗中具有重要的研究价值和应用前景。华美生物所提供的Fish Cortisol ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鱼类血清、血浆、组织匀浆样本中的COR,其灵敏度为0.0023 ng/mL,检测范围为0.0023 ng/mL-10 ng/mL。
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缩写:COR
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种属:Fish
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样本类型:serum, plasma, tissue homogenates
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检测范围:0.0023 ng/mL-10 ng/mL
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灵敏度:0.0023 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 90 Range % 86-98 1:40 Average % 96 Range % 92-100 1:80 Average % 82 Range % 81-88 1:160 Average % 95 Range % 91-101 -
回收率:
The recovery of fish Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 91 87-96 EDTA plasma (n=4) 98 92-105 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 10 0.207 0.196 0.202 2.5 0.396 0.381 0.389 0.625 0.531 0.575 0.553 0.156 0.948 0.982 0.965 0.039 1.326 1.390 1.358 0.01 1.855 1.996 1.926 0.0023 2.097 2.200 2.149 0 2.367 2.302 2.335 -
数据处理:
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货期:3-5 working days
引用文献
- Defatted Black Soldier Fly Meal in Diet for Grow-Out Gilthead Seabream (Sparus aurata L. 1758): Effects on Growth Performance, Gill Cortisol Level, Digestive Enzyme Activities, and Intestinal Histological Structure F Gai,Aquaculture Research,2023
- Stress Response to Entrainment Flow Speed near Pump Inlet Fish Screens in Two Model Teleost Species, Anguilla anguilla and Oncorhynchus mykiss A Miccoli,Fishes,2023
- Effects of Stocking Density, Size, and External Stress on Growth and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) in a Commercial RAS B Baßmann,Fishes,2023
- Chronic exposure to environmental concentrations of harmful algal bloom-forming dinoflagellates induces oxidative stress and reduces immune and hepatic functions in red seabream MN Haque,Comparative biochemistry and physiology. Toxicology & pharmacology,2023
- Primary and Secondary Physiological Stress Responses of European Sea Bass (Dicentrarchus labrax) Due to Rearing Practices under Aquaculture Farming Conditions in M’diq Bay, Moroccan Mediterranean: The Case of Sampling Operation for Size and Weight Measure S Cheyadmi,Life,2023
- Effects of the Antidepressant Amitriptyline on Juvenile Brown Trout and Their Modulation by Microplastics H Schmieg,Toxics,2022
- Ammonia toxicity in Nile tilapia: Potential role of dietary baicalin on biochemical profile, antioxidant status and inflammatory gene expression GE Elshopakey,Aquaculture Reports,2022
- Growth and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) under Dietary Supplementation with Mixed-Layer Clay Mineral Montmorillonite-Illite/Muscovite in Commercial Aquaculture HW Palm,Aquaculture Journal,2022
- Optimization of Eucommia ulmoides Oliver bark extracts by Box-Behnken design and their effects on growth performance, plasma biochemical and immunological parameters, and antioxidant enzyme- and inflammation-related genes expression in Japanese eel, Angui JW Lim,Aquaculture Reports,2022
- Schizochytrium-derived algal oil as replacement for fish oil-effect on growth and smoltification process of Atlantic salmon (Salmo salar L.) DH Blaauw,Nord universitet,2022
- Growth and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) under Dietary Supplementation with Mixed-Layer Clay Mineral Montmorillonite-Illite/Muscovite in Commercial Aquaculture HW Palm,Aquaculture Journal,2022
- Rapid response of osmotic stress transcription factor 1 (OSTF1) expression to salinity challenge in gills of marine euryhaline milkfish (Chanos chanos) YT Lin,PloS one,2022
- Differential effects of two seawater transfer regimes on the hypoosmoregulatory adaptation, hormonal response, feed efficiency, and growth performance of juvenile steelhead trout M Lee,Aquaculture Reports,2022
- Effect of Temperature Fluctuation and Nutritional Status on Starry Flounder, Platichthys stellatus, Survival and Adaptive Physiological Response SS Kim,Journal of Marine Science and Engineering,2021
- Repurposing of Omarigliptin as a Neuroprotective Agent Based on Docking with A2A Adenosine and AChE Receptors, Brain GLP-1 Response and Its Brain/Plasma Concentration Ratio after 28 Days Multiple Doses in Rats Using LC-MS/MS Haidy E. Michel,Molecules,2021
- Effekten av lufteksponering og trenging på primære og sekundære stressresponser hos atlantisk laksesmolt (Salmo salar L.) BH Ottestad,Nord,2020
- Constant and intermittent hypoxia modulates immunity, oxidative status, and blood components of red seabream and increases its susceptibility to the acute toxicity of red tide dinoflagellate SE Nam,Fish & Shellfish Immunology,2020
- Assessment of cadmium toxicity and its possible effects on goldfish (Carassius auratus ), employing microscopy and biochemical techniques Z Noor,Microsc Res Tech,2020
- Possible role of C 60 fullerene in the induction of reproductive toxicity in the freshwater fish, Anabas testudineus (Bloch, 1792) N Sumi,Environmental Science and Pollution Research,2020
- Impact of the antidepressant citalopram on the behaviour of two different life stages of brown trout M Ziegler,PeerJ,2020
- Evaluating the lucrative role of probiotics in the aquaculture using microscopic and biochemical techniques Noor Z, et al,Microscopy Research and Technique,2019
- Dietary supplementation of astaxanthin enhances hemato-biochemistry and innate immunity of Asian seabass, Lates calcarifer (Bloch, 1790) Keng ChinLim, et al,Aquaculture,2019
- Waterborne zinc pyrithione modulates immunity, biochemical, and antioxidant parameters in the blood of olive flounder Min BH, et al,Fish & Shellfish Immunology,2019
- Cortisol regulation of Na+, K+-ATPase β1 subunit transcription via the pre-receptor 11β-hydroxysteroid dehydrogenase 1-like (11β-Hsd1L) in gills of hypothermal freshwater milkfish, Chanos chanos Hu YC,The Journal of Steroid Biochemistry and Molecular Biology,2019
- Cortisol regulation of Na+, K+-ATPase β1 subunit transcription via the pre-receptor 11β-hydroxysteroid dehydrogenase 1-like (11β-Hsd1L) in gills of hypothermal freshwater milkfish, Chanos chanos Hu YC,The Journal of Steroid Biochemistry and Molecular Biology,2019
- Waterborne manganese modulates immunity, biochemical, and antioxidant parameters in the blood of red seabream and black rockfish Do JW, et al,Fish & shellfish immunology,2019
- Effects of Ortho-Phosphate on Growth Performance, Welfare and Product Quality of Juvenile African Catfish (Clarias gariepinus) Sebastian M. Strauch, et al,Fishes,2019
- Effect of plant density in coupled aquaponics on the welfare status of African catfish, Clarias gariepinus Bjorn Babmann, et al,Journal of the World Aquaculture Society,2018
- Waterborne Phenanthrene Modulates Immune, Biochemical, and Antioxidant Parameters in the Bloods of Juvenile Olive Flounder Niamul Haque.et al,Toxicology and Environmental Health Sciences,2018
- Effects of blue light spectra on retinal stress and damage in goldfish (Carassius auratus) Song JA.et al,Fish Physiol Biochem,2018
- Stress and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) in a Coupled Aquaponic System Bj枚rn Ba?mann.et al,Water Environ Res.,2017
- Effects of Various Light Spectra on Physiological Stress and DNA Damage by Thermal Stress in Juvenile Rock Bream (Oplegnathus fasciatus). Marzia Pasquali1.et al,Ocean & Polar Research ,2017
- Hepatoprotective efficacy of Spirulina platensis against lead-induced oxidative stress and genotoxicity in catfish; Clarias gariepinus Sayed AEH.et al,Ecotoxicol Environ Saf.,2017
- Effect of green light spectra on the reduction of retinal damage and stress in goldfish, Carassius auratus. Song JA.et al,Biochem Biophys Res Commun.,2016
- Profiles of hypothalamus?pituitary?interrenal axis gene expression in the parr and smolt stages of rainbow trout, Oncorhynchus mykiss: Effects of recombinant aquaporin 3 and seawater acclimation Young Jae Choi.et al,Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology,2015
- Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015
- Effects of waterborne selenium on toxic and physiological stress response in goldfish, Carassius auratus Choi Y J.et al,Molecular & Cellular Toxicology,2015
- Effects of melatonin and green-wavelength LED light on the physiological stress and immunity of goldfish, Carassius auratus, exposed to high water temperature Jung SJ.et al,Fish Physiol Biochem.,2016
- Changes of physiological rhythms of N-methyl-d-aspartate receptors in the chum salmon Oncorhynchus keta: effect of seawater acclimation during the parr-smolt transformation Young Jae Choi.et al,Biological Rhythm Research,2016
- The Expression of Leptin, Estrogen Receptors, and Vitellogenin mRNAs in Migrating Female Chum Salmon, Oncorhynchus keta: The Effects of Hypo-osmotic Environmental Changes Young Jae Choi et al, Asian Australas. J. Anim. Sci.,2014
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问答及客户评论
该试剂盒是否测量总皮质醇水平或仅测量游离/未结合的皮质醇? 我的实验样本要使用斑马鱼组织匀浆,但我在说明书中没有看到有关检测总皮质醇还是只检测游离皮质醇的任何信息。
1.这个试剂盒有PBS吗?里面的缓冲是PBS吗?Tris-HCl能否用作洗涤溶液?在初步研究中,我的实验室写明他们使用了Tris-HCl,但我不知道为什么他们选择它而不是PBS。 2.获取组织匀浆时,您是否有任何研磨组织的建议?
靶点详情
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最新研究进展:COR(Cordoba)是一种脱氧核糖核酸(DNA)结合蛋白,它与DNA结合并参与细胞核内基因表达调控。最近的研究表明,COR蛋白在干细胞的维持和分化中起着重要作用。此外,COR还被发现参与一些人类疾病的发生,例如神经退行性疾病和某些癌症。在细胞增殖和肿瘤发生中,COR蛋白的过度表达可能会对肿瘤的形成和发展产生影响。
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