TFRC

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2μg/mL can bind Anti-TFRC recombinant antibody (CSB-RA023441MA1HU)， the EC₅₀ is 3.305-8.220 ng/mL.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human TFRC at 2 μg/ml can bind Human TF (CSB-MP023412HU). The EC₅₀ is 33.20-40.09 ng/mL.

TFRC Recombinant Monoclonal Antibody (CSB-RA023441MA1HU) captured on Protein A Chip can bind Recombinant Human TFRC protein with an affinity constant of 0.117 nM as detected by MetaSPR Assay (WeSPRTM 200).

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, HEK293 whole cell lysate, MCF7 whole cell lysate
All lanes: TFRC antibody at 1:5000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa

IHC image of CSB-PA07219A0Rb diluted at 1:150 and staining in paraffin-embedded Human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-PA07219A0Rb diluted at 1:150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of HepG2 cell with CSB-PA07219A0Rb at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).