PVR

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
FACS assay shows that Human PVR can bind to HEK293F cell overexpressing human TIGIT.

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: Poliovirus Receptor antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa

IHC image of CSB-RA567944A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.42% DAB.