LIFR

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized human LIF (CSB-MP012928HU) at 2 μg/ml can bind human LIFR, the EC₅₀ is 11.68-15.52 ng/ml.

Immunofluorescence staining of A549 cells with CSB-PA012929LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: MCF7 whole cell lysate,Rat Brain tissue lysate
All lanes: LIFR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123 kDa
Observed band size: 123 kDa