CD34

IHC image of CSB-RA004926A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot
Positive WB detected in: Mouse brain tissue
All lanes: CD34 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41, 36 kDa
Observed band size: 41 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA10509A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA10509A0Rb at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA10509A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of PC-3 cells using CSB-PA10509A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)