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E-Tag Monoclonal Antibody

Datasheet
  • 货号:
    CSB-MA000151M0m
  • 规格:
    ¥600
  • 图片:
    • Western Blot
      Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Western Blot
      Positive WB detected in: E-tagged fusion protein
      All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

       

    • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
      Antigen at dilution of 1µg/ml
      E-Tag antibody at (0-0.15µg)
      Goat polyclonal to Mouse IgG at 1/50000 dilution

       

    • Western Blot
      Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 30, 68, 55, 67-157kDa
      Observed band size: 30, 68, 55, 67-157kDa

  • 其他:

产品详情

  • 产品描述:

    The monoclonal anti-E-Tag (mouse IgG1 isotype) is produced from the hybridoma fused by the mouse myeloma cells and splenocytes from the immunized mouse. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH and can secret the E-Tag antibody. This unconjugated E-Tag antibody is purified through protein G with a purity of more than 95%. It only reacts with samples containing E-Tag. And it has been amenable for ELISA, WB, and IP applications.

    The E-tag is a popular epitope tag containing 13 amino acids. It is commonly expressed or detected with the protein of interest as an N- or C-terminal fusion. Anti-E tag antibodies are amenable for the detection, isolation, and purification of E-tagged proteins.

  • 别名:
    E-Tag
  • 宿主:
    Mouse
  • 反应种属:
    All
  • 免疫原:
    GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal Antibody
  • 抗体亚型:
    IgG1
  • 纯化方式:
    >95%, Protein G purified
  • 克隆号:
    11H12B3
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IP
  • 推荐稀释比:
    Application Recommended Dilution
    ELISA 1:4000-1:8000
    WB 1:1000-1:320000
    IP 1μg-5μg
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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