Recombinant Mouse Vimentin (Vim)
In Stock-
中文名称:小鼠Vim重组蛋白
-
货号:CSB-EP025857MO
-
规格:¥2328
-
图片:
-
其他:
产品详情
-
纯度:Greater than 85% as determined by SDS-PAGE.
-
基因名:
-
Uniprot No.:
-
别名:VimVimentin
-
种属:Mus musculus (Mouse)
-
蛋白长度:Full Length of Mature Protein
-
来源:E.coli
-
分子量:61.0 kDa
-
表达区域:2-466aa
-
氨基酸序列STRSVSSSSYRRMFGGSGTSSRPSSNRSYVTTSTRTYSLGSALRPSTSRSLYSSSPGGAYVTRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAESTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHDEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFALEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPTFSSLNLRETNLESLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白标签:N-terminal 10xHis-tagged and C-terminal Myc-tagged
-
产品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
缓冲液:Tris-based buffer,50% glycerol
-
储存条件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
-
保质期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
货期:3-7 business days
-
注意事项:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
-
产品描述:
Just like other recombinant proteins, the production of this recombinant Mouse Vim protein began with appropriate cDNA and PCR methods, and then the Vim expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into E.coli for the expression of the recombinant Mouse Vim protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 85%+.
Vim is a gene encoding a protein named vimentin in mouse. Other specieses, such as human, also have vimentin protein. It has been reported that vimentin, a cytoskeleton filament that is expressed only in mesenchymal cells after birth. It is re-expressed in epithelial cells in vivo under pathological conditions and in vitro in primary culture. This protein is involved in many biological processes, such as astrocyte development, regulation of mRNA stability, regulation of mRNA stability, etc. Recombinant vim A can be used to researching the interaction of proteins, such as plectin and vim.
-
Datasheet & COA:Please contact us to get it.
相关产品
靶点详情
-
功能:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
-
基因功能参考文献:
- The Listeria monocytogenes virulence factor InlF was found to bind vimentin and was necessary for optimal bacterial colonization of the brain. PMID: 29487235
- MAGE-G1 interacted with fascin 1 or vimentin in P19 cells after a 6-day retinoic acid-induced neuronal differentiation. PMID: 28374796
- Results found that the absence of vimentin impairs spontaneous endothelial differentiation in vitro and have furthering the understanding of the regulators of differentiation. PMID: 27480130
- Protein phosphatase 1 is a key protein serine/threonine phosphatase that controls vimentin Ser-56 dephosphorylation in smooth muscle. PMID: 27457922
- These findings identify vimentin as a positive regulator of stemness in the developing mouse mammary gland and in breast cancer cells. PMID: 28947532
- This study is the first to show that vimentin has an important role in tumor metastasis in vivo in the setting of pre-diabetes and endogenous hyperinsulinemia. PMID: 27568979
- These findings identify two specific sites on vimentin that are phosphorylated by Cadmium. PMID: 28450285
- The expression level of vimentinin liver cirrhotic tissues were significantly higher than that in chronic hepatitis tissues. PMID: 27538444
- both arthritis-susceptible and -resistant mice can generate cellular and humoral immunity to Vim. PMID: 27602574
- vimentin knockout neurons were insensitive to the axonotrophic effects of Clostridium botulinum C3 exoenzyme PMID: 27419376
- These findings suggest that Plk1 regulates smooth muscle contraction by modulating vimentin phosphorylation at Ser-56. PMID: 27662907
- findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition PMID: 26251181
- these findings identify a hereto-unappreciated role for serine-38 phosphorylated vimentin as an important determinant of myofibroblast sensitivity to Withaferin A. PMID: 26186445
- Vimentin expression increased after traumatic brain injury and was positively correlated with edema and neurological impairments. PMID: 26039099
- Annexin, lamin, and vimentin were identified as universal dystrophic markers PMID: 26102067
- Astrocytes deficient of GFAP and vimentin showed decreased Notch signal sending competence and altered expression of Notch signaling pathway-related genes PMID: 26118771
- Absence of GFAP, or both GFAP and vimentin, alters Alzheimer's disease-induced changes in gene expression profile of astrocytes, showing a compensation of the decrease of neuronal support genes and a trend for a higher inflammatory expression profile PMID: 25731615
- Vimentin is not only a major organizing element of the intermediate filament network but is also involved in both binding and uptake of C3 exoenzyme. PMID: 24967582
- Cytokinetic Failure-induced Tetraploidy Develops into Aneuploidy, Triggering Skin Aging in Phosphovimentin-deficient Mice. PMID: 25847236
- Vimentin has a role in regulating activation of the NLRP3 inflammasome PMID: 25762200
- protein(s) that associated with RPTPbeta in response to IGF-I and IGFBP-2 in vascular smooth muscle cells PMID: 25787077
- Data suggest MAP kinase-interacting kinases (Mnk1, Mnk2) regulate cell migration/wound healing, expression of Vim, stability of Vim, and binding of eIF4E (eukaryotic translation initiation factor 4E)/Cyfip1 (cytoplasmic FMR1 interacting protein 1). PMID: 25588502
- This review discusses various novel functions which are now known to be mediated by vimentin, summarizing structure, regulation and roles of vimentin in cell adhesion, migration, angiogenesis, neurite extension, and cancer. PMID: 24387004
- Double-immunostaining experiments with antibodies against Stk33 and vimentin showed a striking colocalization of Stk33 and vimentin in the hypothalamus. PMID: 24057876
- Keratocyte activation and differentiation play a key role in fibrosis, and vimentin, a major structural type III intermediate filament, is a required component of this process. PMID: 24854859
- absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics PMID: 24223940
- We demonstrate, using long-term 4D imaging, that the vimentin intermediate filament establishes mitotic polarity in mammalian cell lines and mediates the asymmetric partitioning of damaged proteins. PMID: 24843142
- defect of mitotic vimentin phosphorylation causes microophthalmia and cataract PMID: 24142690
- Vimentin synthesis initiates during a differentiation process of trophoblast giant cells and continues throughout the stage of vascular TGC. PMID: 23664004
- These data suggest that vimentin is required for the minute virus of mice life cycle, presenting possibly a dual role: (1) following virus escape from endosomes and (2) during endosomal trafficking PMID: 23838001
- Data indicate interaction of PPARgamma with vimentin in the cytosolic compartment, in which vimentin regulating the turnover rate of PPARgamma, which further regulate genomic or non-genomic activities through the regulation of PPARgamma protein degradation. PMID: 23297177
- involvement of vimentin in migration processes at different stages of development and try to resolve current contradictions concerning the role of vimentin in various events of cell migration PMID: 23885566
- we propose a novel regulatory mechanism of IP3R1 activity by type III intermediate filament vimentin PMID: 22929228
- Data indicate that vimentin intermediate filaments must coalesce at mature focal adhesions for efficient endoplasmic spreading. PMID: 23115305
- These data show for the first time that decorin has an impact on the biology of alpha2beta1 integrin and the vimentin intermediate filament system. PMID: 23226541
- overexpression of mesangial integrin alpha1 and podocyte vimentin and integrin alpha3 may be important features of glomerular Alport disease PMID: 23236390
- expression detected in the mylohyoid muscle and surrounding tissues at embryonic day 12 PMID: 22476899
- TGFbeta stimulates vimentin production via PI3K-Akt-mTOR signaling, which leads to suppression of ATF4-dependent Ocn transcription and osteoblast differentiation. PMID: 22952236
- vimentin ablation results in defective steroidogenesis PMID: 22535769
- vimentin is a regulator of NRG1 type III function and peripheral nerve myelination PMID: 22357929
- Endothelial cell surface vimentin binding peptide induces angiogenesis under hypoxic/ischemic conditions. PMID: 21803052
- absence of Zmpste24 profoundly alters the processing of the cytoskeletal protein vimentin PMID: 21828285
- Corneal antifibrotic switch identified in genetic and pharmacological deficiency of vimentin. PMID: 22117063
- Data demonstrated that Abeta toxic species cross the plasma membrane, accumulate in cells and bind to a variety of internal proteins, vimentin (cytoskeleton), cathepsin D (lysosomes), GRP-78 (endoplasmic reticulum and associated membranes). PMID: 21966382
- study reports that vimentin filaments associate with collagen mRNAs in a 5'stem-loop sequence and LARP6-dependent manner and stabilize collagen mRNAs PMID: 21746880
- When vimentin organization is disrupted by a dominant-negative mutant or by silencing, there is a loss of polarity. These findings demonstrate an antagonistic relationship between vimentin intermediate filaments (VIF) and the formation of lamellipodia. PMID: 21346197
- Mature focal adhesions and their derivative fibronectin fibril-aligned fibrillar adhesions (FbAs) serve as docking sites for vimentin intermediate filaments (IFs) in a plectin isoform 1f (P1f)-dependent manner. PMID: 20702585
- Increases in post-traumatic vimentin mRNA levels in the cortex and in the hippocampus appear together with vimentin immunoreactivity in astrocytes starting one day after severe trauma. PMID: 20479526
- The interaction of hormone-sensitive lipase with vimentin, and its hormonal dependence, was confirmed by coimmunoprecipitation. PMID: 20143880
- Sol-gel polymerization of tetraethoxysilane proceeded preferentially on the surface of intermediate filaments assembled from vimentin protein in vitro, resulting in silica-coated fibres. PMID: 19656809
显示更多
收起更多
-
亚细胞定位:Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.
-
蛋白家族:Intermediate filament family
-
组织特异性:Detected in eye lens fiber cells (at protein level). Expressed in retinal lens epithelial cells (at protein level).
-
数据库链接:
Most popular with customers
-
Recombinant Human Heat-stable enterotoxin receptor (GUCY2C), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Rabbit Tissue factor pathway inhibitor (TFPI) (Active)
Express system: Mammalian cell
Species: Oryctolagus cuniculus (Rabbit)
-
Recombinant Human Desmoglein-3 (DSG3), partial (Active)
Express system: Baculovirus
Species: Homo sapiens (Human)
-
Recombinant Human Alkaline phosphatase, germ cell type (ALPG) (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Human Oncostatin-M (OSM), partial (Active)
Express system: Mammalian cell
Species: Homo sapiens (Human)
-
Recombinant Mouse Cytotoxic and regulatory T-cell molecule (Crtam), partial (Active)
Express system: Mammalian cell
Species: Mus musculus (Mouse)
-
Recombinant Human Kidney-associated antigen 1(KAAG1) (Active)
Express system: E.coli
Species: Homo sapiens (Human)