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Recombinant Mouse Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(Ppp2ca)

  • 货号:
    CSB-YP018559MO
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 货号:
    CSB-EP018559MO
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 货号:
    CSB-EP018559MO-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 货号:
    CSB-BP018559MO
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 货号:
    CSB-MP018559MO
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    Ppp2ca
  • Uniprot No.:
  • 别名:
    Ppp2ca; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; EC 3.1.3.16
  • 种属:
    Mus musculus (Mouse)
  • 蛋白长度:
    Full length protein
  • 表达区域:
    1-309
  • 氨基酸序列
    MDEKLFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV TRRTPDYFL
  • 蛋白标签:
    The following tags are available.
    N-terminal His-tagged
    Tag-Free
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3.
  • 基因功能参考文献:
    1. miR-203-3p target Ppp2ca aggravates status epilepticus model in mice. PMID: 30121817
    2. Data (including data from studies in knockout/transgenic mice) suggest Ppp2ca supports cortical neuronal growth and cognitive function via regulating p73/Gls2 signal transduction in neurons of hippocampus. Ppp2ca gene knock-out results in embryonic cortical atrophy with learning/memory deficits. (Ppp2ca = protein phosphatase 2a catalytic subunit alpha isoform; p73 = transformation related protein 73; Gls2 = glutaminase-2) PMID: 29274472
    3. Results indicate that Dlx5 and Runx2 are critical factors for the upregulated Osterix expression in shPP2A cells, which is considered to be important for the accelerated osteoblast differentiation in these cells. PMID: 29068100
    4. Data (including data from studies in transgenic mice) suggest that Esr1 signaling promotes activation of Pp2a; here, central activation of Pp2a during estrogen replacement therapy is involved in prevention of menopause-induced obesity and glucose intolerance (that is, induced by lack of membrane-initiated Esr1 signaling). (Esr1 = estrogen receptor 1; Pp2a = protein phosphatase 2A) PMID: 29764860
    5. The data also suggest that H2A.Z restricts transcription, which is moderated by ANP32e at the promoter and gene bodies of expressed genes. Thus, ANP32e, through inhibition of PP2A, is required for nucleosomal inclusion of H2A.Z and the regulation of gene expression PMID: 29524612
    6. PP2A is a target of piperine, which induces autophagy in a rotenone-induced Parkinson's disease model PMID: 27572322
    7. We describe a novel mechanism of signal transduction enriched in medium spiny neurons of striatum that likely mediates effects of the neurotransmitter dopamine acting on these cells. We find that the protein ARPP-16, which is highly expressed in striatal medium spiny neurons, acts as a selective inhibitor of certain forms of the serine/threonine protein phosphatase, PP2A, when phosphorylated by the kinase, MAST3. PMID: 28167675
    8. data show that Fyn kinase is activated after TLR4 triggering and exerts an important negative control on LPS-dependent TNF production in mast cells controlling the inactivation of PP2Ac and activation of PKCalpha/beta necessary for the secretion of TNF by VAMP3(+) carriers PMID: 27183589
    9. endogenous siRNA (PTEN-sh-3p21) cleaved from PTEN-sh within PTEN mRNA 3'UTR modulates PPP2CA and PTEN at the post-transcriptional level in liver cells PMID: 27133296
    10. PP2A activation both limited and prevented inflammation and tissue injury in two direct injury models of Acute respiratory distress syndrome. PMID: 27638902
    11. critical role in the proliferation and metastasis of osteosarcoma cells PMID: 27617401
    12. PP2A regulates kinetochore-microtubule attachment during meiosis I in oocyte. PMID: 27096707
    13. Study demonstrates that PPP2Ac-alpha plays important roles in both hair follicle and skin development. PMID: 27213341
    14. these results suggest that inhibiting PP2Ac nitration using a mimic peptide is a potential preventive strategy for Endothelial-to-mesenchymal transition in renal fibrosis PMID: 26805394
    15. Data suggest a critical role for the I2PP2A protein (SET)-protein phosphatase-2A (PP2A) signaling axis in Pten phosphohydrolase PMID: 26563471
    16. that loss of glucocerebrosidase function may contribute to SNCA accumulation through inhibition of autophagy via PPP2A inactivation PMID: 26378614
    17. Data indicate that protein phosphatase PP2A is required for the function of T(reg) cells and the prevention of autoimmunity. PMID: 26974206
    18. data identify a molecular mechanism linking PP2A to the development of AD-related cognitive impairments that might be therapeutically exploited to target selectively the pathological effects caused by elevated Abeta levels in AD patients PMID: 26951658
    19. PP2A has a role in mediating oxidative stress induced apoptosis in osteoblasts PMID: 26538836
    20. Our results show that D2R controls renal inflammation, at least in part, by modulation of the Akt pathway through effects on PP2A activity/expression. PMID: 26290374
    21. Concurrent mTORC1 inactivation and PP2A-B55alpha stimulation fuel ULK1-dependent autophagy. PMID: 26310906
    22. metabolic remodeling in PP2Acalpha deficiency and chronic pressure overload mouse hearts PMID: 26497085
    23. Liver-specific Ppp2calpha knockout mice showed enhanced glucose homeostasis and increased insulin sensitivity by activation of insulin signaling through Akt. PMID: 25888638
    24. Loss of Ppp2ca expression is associated with infertility in male. PMID: 25628439
    25. Data indicate that the regulation of mTORC1 signaling and protein phosphatase 2 PP2A activity by ciliary transport plays key roles in PDGF-AA/alphaalpha signaling. PMID: 25392303
    26. Arsenic may initiate endothelial dysfunction, resulting in vascular leakage in response to vasoactive agents. PMID: 24448713
    27. Loss of TFF1 promotes beta-catenin activation and gastric tumorigenesis through regulation of PP2A, a major regulator of AKT-GSK3beta signaling. PMID: 25107557
    28. NOSIP mediates the monoubiquitination of the PP2A catalytic subunit and the loss of NOSIP results in an increase in PP2A activity in craniofacial tissue in NOSIP knockout mice. PMID: 25546391
    29. REDD1 may act as a targeting protein for the catalytic subunit of PP2A PMID: 25056877
    30. Data suggest thet 67-kDa laminin receptor-dependent protein phosphatase 2A (67LR/PP2A) may be a promising therapeutic target for melanomas. PMID: 25294877
    31. While AMPK activation in motor neurons correlateswith progressionin mutant SOD1-mediated disease, AMPK inactivation mediated by PP2A is associated withmutant TDP-43-linked amyotrophic lateral sclerosis. PMID: 24595038
    32. enhanced SK2 channel control over NMDAR contributes to LTP impairment via increased PP2A activity following contextependent sensitization to morphine. PMID: 23735878
    33. beta-N-methylamino-l-alanine leads to tau pathology by inhibiting PP2A through the activation of mGluR5, the consequent release of PP2Ac from the mGluR5-PP2A complex, and its phosphorylation at Tyr(307) by Src PMID: 24395787
    34. Protein phosphatase 2A dephosphorylates phosphoserines in nucleocytoplasmic shuttling and secretion of HMGB1. PMID: 23782844
    35. PP2A participates in SNAP-25 dephosphorylation through calcium-dependent and calcium-independent mechanisms but is not involved in GAP-43 dephosphorylation. PMID: 23376809
    36. SET/PP2A system regulates testosterone production in ovarian follicles in vitro. PMID: 23628604
    37. The effects of ethanol on AMPK and PP2A may result in activation of ChREBP, providing another potential mechanism for ethanol-induced hepatic steatosis. PMID: 23266705
    38. by promoting the inflammatory capacity of T cells, PP2Ac dysregulation contributes to the pathogenesis of systemic lupus erythematosus PMID: 23918926
    39. Studies indicate that phenobarbital binding to EGFR inhibits distal signaling to derepress RACK1-facilitated dephosphorylation of constitutive active androstane receptor (CAR) by PP2A, and activates the transcription of Cyp2B10. PMID: 23652202
    40. constitutively active PP2Ac disrupts the signal-promoting TLR4-MyD88 complex and broadly suppresses multiple proinflammatory/proapoptotic pathways. PMID: 23434512
    41. At early developmental stages, when axons are growing, the protein phosphatase PP2A interacted with and dephosphorylated Dock6, thereby increasing the activity of Dock6 PMID: 23462102
    42. ginsenoside Rb1 protected mice against aluminum-induced toxicity. The possible mechanism may be its role in preventing tau hyperphosphorylation by regulating p-GSK3 and PP2A level PMID: 23219964
    43. PP2A is inactivated by zinc via Src dependent phosphorylation at tyrosine 307. PMID: 22892311
    44. Semaphorin 3A impinged upon the basal activity of the serine phosphatase PP2A and disrupted PP2A interaction with VE-cadherin, leading to cell-cell junction disorganization and increased permeability. PMID: 22685328
    45. This study provides evidence that CaBP4 is dephosphorylated by PP2A in the retina. PMID: 23341017
    46. These results indicate that PP2A Calpha and its activity play a negative role in osteoblast differentiation and Osterix is a key factor responsible for regulating the expressions of Bsp and OCN during PP2A Calpha-mediated osteoblast differentiation. PMID: 23042641
    47. CKIP-1 was found to be an inhibitor of cardiac hypertrophy by upregulating the dephosphorylation of HDAC4 through the recruitment of protein phosphatase 2A. PMID: 23151343
    48. PP2A deficits observed in Alzheimer disease may cause Abeta accumulation via hindering the astrocytes migration. PMID: 22729898
    49. our results suggest that Asp664 cleavage of APP leads to tau hyperphosphorylation at specific epitopes by modulating PP2A activity PMID: 23020770
    50. DUSP6 and PP2A participate in the regulation of melanogenesis PMID: 22544520

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  • 亚细胞定位:
    Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族:
    PPP phosphatase family, PP-1 subfamily
  • 数据库链接: