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Recombinant Human SARS coronavirus Spike glycoprotein(S),partial (Active)

In Stock
  • 货号:
    CSB-MP348663HQE
  • 规格:
    ¥27960
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml. Biological Activity Assay
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml. Biological Activity Assay
  • 其他:

产品详情

  • 纯度:
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 2 μg/ml can bind Paguma larvata ACE2 (CSB-MP684964PAL), the EC50 is 5.056-7.559 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD at 5 μg/ml can bind human ACE2 (CSB-MP866317HU), the EC50 is 7.941-10.49 ng/ml.
  • 基因名:
    S
  • Uniprot No.:
  • 别名:
    (S glycoprotein)(E2)(Peplomer protein)(Spike protein S1)(Spike protein S2)
  • 分子结构:
  • 种属:
    Human SARS coronavirus (SARS-CoV) (Severe acute respiratory syndrome coronavirus)
  • 蛋白长度:
    Partial
  • 来源:
    Mammalian cell
  • 分子量:
    30.0 kDa
  • 表达区域:
    306-527aa
  • 氨基酸序列
    RVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNF
  • 蛋白标签:
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    3-7 business days
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述:

    Human SARS-CoV spike (S) protein amino acids 306-527 linked an N-terminal 10xHis-tag by the Thrombin linker, as well as a C-terminal Myc tag, was expressed in mammalian cells. The product is the recombinant human SARS-CoV S protein. This SARS-CoV S protein is an active protein, whose activity has been determined through functional ELISA by binding to ACE2 of Paguma larvata and human, with EC50 values of 5.056-7.559 ng/ mL and 7.941-10.49 ng/ mL, respectively. Its purity is high (>95%, SDS-PAGE) and endotoxin is low (<1.0 EU/ug protein, LAL method). Due to the glycosylation, it has an apparent molecular weight of 36 kDa on the gel. It is in stock now.

    The SARS-CoV is an enveloped, single, and positive-stranded RNA virus. Its S protein is made up of the S1 subunit and S2 subunit. The S1 subunit contains a receptor-binding domain responsible for the engagement with the host cell receptor ACE2. The S2 subunit mediates the virus-host cell membrane fusion. The S protein plays important role in the induction of neutralizing-antibody and T-cell responses, and protective immunity during SARS-CoV infection.

  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.; attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.; mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.; Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
  • 亚细胞定位:
    Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein.
  • 蛋白家族:
    Betacoronaviruses spike protein family