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APC Antibody

Datasheet
  • 货号:
    CSB-RA951649A0HU
  • 规格:
    ¥2200
  • 图片:
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate
      All lanes: APC antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 312, 301, 309 kDa
      Observed band size: 160 kDa
    • IHC image of CSB-RA951649A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他:

产品详情

  • Uniprot No.:
  • 基因名:
    APC
  • 别名:
    Adenomatous polyposis coli protein (Protein APC) (Deleted in polyposis 2.5), APC, DP2.5
  • 反应种属:
    Human, Rat
  • 免疫原:
    A synthesized peptide derived from human APC
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    Rabbit IgG
  • 纯化方式:
    Affinity-chromatography
  • 克隆号:
    5F2
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, WB, IHC
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能:
    Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.
  • 基因功能参考文献:
    1. elevated expression of miR-494 promotes cell proliferation and tumorigenesis in CRC by suppressing the expression of APC, an inhibitor of beta-catenin signaling. These findings uncover a novel molecular mechanism for the hyperactivation of the Wnt/beta-catenin signaling pathway in CRC. PMID: 29304823
    2. Study reveal an unexpected role of APC in the directional spread of HIV-1 by promoting the directional assembly of viral components at virological synapses, thereby facilitating cell-to-cell viral transmission. PMID: 28134256
    3. Because of low sensitivity, APC gene promoter methylation in serum was not suitable for breast cancer (BC) screening. However, as specificity was very high, detection of serum APC gene promoter methylation could be used as tool to confirm BC. PMID: 29297603
    4. Multivariate analyses revealed that the PIK3CA mutation and clinical T stage were independent favorable prognostic factors (hazard ratio 0.34, 95% confidence interval: 0.12-0.96, p = 0.042). PIK3CA mutations were significantly associated with APC alterations (p = 0.0007) and BRAF mutations (p = 0.0090). PMID: 30115035
    5. A novel APC frameshift mutation has been identified in a large Chinese family with familial adenomatous polyposis. PMID: 29901124
    6. In the two wild type (WT) cases, two novel alterations were detected: a complex deletion of APC and a pathogenic mutation of LAMTOR2. Focusing on WT DT subtype, deep sequencing of CTNNB1, APC and LAMTOR2 was conducted on a retrospective series of 11 WT DT using a targeted approach PMID: 29901254
    7. This study demonstrates a prognostic role for APC. PMID: 27302369
    8. There is a certain correlation between the APC gene and ovarian tumors, and the APC gene mediates the apoptosis of tumor cells through the MDR-1/CLCX-1 signaling pathway. PMID: 29921377
    9. Its mutation is identified in duodenal adenoma and it involves in development of duodenal cancers. PMID: 29525853
    10. We have investigated if the initial source of intratumoral heterogeneity is consequent to multiple independent lineages derived from different crypts harboring distinct truncal APC and driver KRAS mutations, thus challenging the prevailing monoclonal monocryptal model. PMID: 28645942
    11. methylation-dependent silencing of the APC gene promoter 1A is a mechanism that contributes to the activation of Wnt signaling pathway in cervical cancer cells infected by high risk HPV16. PMID: 29115417
    12. miR-3607 contributes to lung cancer cell proliferation by inhibiting APC. PMID: 28866416
    13. USP7 depletion in APC-mutated colorectal cancer inhibits Wnt activation by restoring beta-catenin ubiquitination, drives differentiation, and suppresses xenograft tumor growth. PMID: 29045831
    14. Data suggest that the concurrent mutations of the adenomatous polyposis coli protein (APC) and mutL protein homolog 1 (MLH1) genes probably underline the familial adenomatous polyposis (FAP) in the pedigree. PMID: 29419868
    15. The expression of APC-DeltaC in colon cells reduces the accumulation of mitotic cells upon PLK1 inhibition, accelerates mitotic exit and increases the survival of cells with enhanced chromosomal abnormalities. PMID: 29549256
    16. our results suggest that the amount of APC expression is the rate-limiting factor for the constitution of beta-catenin destruction complexes. PMID: 28810742
    17. APC defines Treg differentiation and anti-inflammatory function through microtubule-mediated NFAT localization. PMID: 28978472
    18. Data show that tumor suppressor adenomatous polyposis coli (APC) loss results in up-regulation of IL-6 signal transducer (IL-6ST/gp130), thereby activating YAP (YY1-associated) protein (YAP) which are simultaneously up-regulated in the majority of colorectal cancer (CRC). PMID: 28130546
    19. The results indicate that APC promoter hypermethylation is an early event in carcinogenesis of CRC, could be a valuable diagnostic marker for early-stage CRC. APC methylation is not significantly associated with overall survival in patients with CRC. PMID: 28515349
    20. the promoters of WIF1, NLK, and APC are highly methylated in the nasopharyngeal cancers (NPC) and gastric carcinoma (GC) cell lines, and the 3 genes are also regulated by miR-BART19-3p expressed by Epstein-Barr virus (EBV); expression of the WIF1, APC, and NLK genes is strongly affected by hypermethylation, and in EBV-associated tumors, the 3 genes are also affected by miR-BART19-3p PMID: 28543390
    21. The model we propose is a variation of the currently existing model and hypothesizes that, in a subgroup of colorectal carcinomas, K-ras mutation may precede APC inactivation, representing the earliest driving force and, probably, an early biomarker of colorectal carcinogenesis. PMID: 28652417
    22. We conclude that among multiple genomic alterations in CRC, strongest associations with clinical outcome were observed for common mutations in APC. PMID: 27729614
    23. Studies reveal that the proportion of APC promoter 1A methylation in non-small cell lung cancer (NSCLC) tissues was higher than in autologous controls, indicating that promoter 1A methylation of the APC gene may play an important role in NSCLC carcinogenesis. [meta-analysis] PMID: 28497891
    24. E-cadherin inhibits beta-catenin in the context of disruption of the APC-destruction complex, and that this function is also EC1 domain dependent. Both binding functions of E-cadherin may be required for its tumour suppressor activity. PMID: 27566565
    25. analysis of the largest deletion of the APC gene in the Chinese population associated with familial adenomatous polyposis in a five generation family PMID: 27391059
    26. APC promoter methylation was associated with breast cancer risk, and it could be a valuable biomarker for diagnosis, treatment and prognosis of breast cancer (Meta-Analysis) PMID: 27191268
    27. A novel APC promoter 1B deletion is associated with familial adenomatous polyposis in generations of a large Italian family. PMID: 28791770
    28. Study is the first to demonstrate that EphB6 overexpression together with Apc gene mutations may enhance proliferation, invasion and metastasis by colorectal epithelial cells. PMID: 27145271
    29. Promoter methylation was detected in 30.67% breast cancer tissues and was associated with low histological grade. PMID: 28164568
    30. Utilizing zebrafish to examine the genetic relationship between MPC1 and Adenomatous polyposis coli (APC), a key tumor suppressor in colorectal cancer, the authors found that apc controls the levels of mpc1 and that knock down of mpc1 recapitulates phenotypes of impaired apc function including failed intestinal differentiation. PMID: 28397687
    31. Multiple pilomatrixomas in a survivor of WNT-activated medulloblastoma leading to the discovery of a germline APC mutation and the diagnosis of familial adenomatous polyposis PMID: 28792655
    32. FZR1 inhibits BRAF oncogenic functions via both APC-dependent proteolysis and APC-independent disruption of BRAF dimers, whereas hyperactivated ERK and CDK4 reciprocally suppress APC(FZR1) E3 ligase activity PMID: 28174173
    33. we present a clinical molecular study of a four generation Chinese family with a novel splice-acceptor site mutation causing Familial adenomatous polyposis PMID: 28423518
    34. Establish a role for APC in coordinating microtubules and actin cytoskeleton at focal adhesions to direct cell migration. PMID: 28663347
    35. Germline mutation in the APC gene is associated with familial adenomatous polyposis. PMID: 28010732
    36. beta-catenin reactivity was noted in all familial adenomatous polyposis-associated Gardner fibromas and in 1/4 APC wild-type cases PMID: 26840078
    37. The rs75612255 C allele and rs113017087 C allele in promoter 1A of APC as well as the rs138386816 T allele and rs115658307 T allele in promoter 1B of APC significantly increased luciferase activity in the human erythromyeloblastoid leukaemia cell line K562. PMID: 28105931
    38. Functional redundancy between Apc and Apc2 regulates tissue homeostasis and prevents tumorigenesis in murine mammary epithelium PMID: 27694902
    39. Finally, we observed that expression of miR-19a significantly correlates with beta-catenin levels in colorectal cancer specimens, and it is associated to the aggressive stage of tumor progression. Thus, our study reveals that miR-17-92 cluster is directly regulated by APC/b-catenin pathway and could be a potential therapeutic target in colon cancers with aberrant APC/b-catenin signaling. PMID: 26804172
    40. Findings show that colorectal Cancer patients-derived cells with short APC mutants were either sensitive or responsive to tankyrase inhibitors corroborating the idea that APC with complete deletion of seven 20-AA repeats could be a predictive biomarker for the sensitivity to tankyrase inhibitors. PMID: 28179481
    41. Our study showed that mutations in the APC gene alter the protein expression and cell cycle regulation in diffuse type gastric adenocarcinoma. PMID: 28576136
    42. Gasdermin C is upregulated by inactivation of Tgfbr2 in the presence of mutated Apc, promoting colorectal cancer cell proliferation. PMID: 27835699
    43. Nine patients with 21- approximately 100 colorectal adenomas (50%) and the two positive controls, showed somatic mosaicism, with identical APC variants in adenomas tested. PMID: 27816598
    44. These result suggested that miR-590-3p can promote osteogenic differentiation via suppressing APC expression and stabilizing beta-catenin. PMID: 27586273
    45. The present findings indicate epigenetic silencing of APC in advanced gallbladder cancer (GBC). The methylation pattern, followed by expression analysis of APC may be suggested for diagnostic, prognostic and therapeutic purposes in GBC in future PMID: 27748282
    46. miR-106a-5p is involved in the invasive behavior of glioblastoma cells and by targeting APC and activating Wnt/beta-catenin pathway. PMID: 27815074
    47. Point Mutations in Exon 1B of APC Reveal Similar mutations in APC promoter 1B occur in rare families with familial adenomatous polyposis PMID: 27087319
    48. Studies suggest that both loss of tumor suppressive function and gain of function of APC mutants play critical roles in colorectal cancers (CRC) tumorigenesis. PMID: 28423402
    49. Loss of heterozygosity of TP53, P16, SMAD4 and APC genes was observed in esophageal adenocarcinoma. PMID: 28376920
    50. miR-582-5P was upregulated in the colorectal cancer specimens and cell lines and targeted the 3' untranslated region of APC directly. PMID: 27595705

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  • 相关疾病:
    Familial adenomatous polyposis (FAP); Hereditary desmoid disease (HDD); Medulloblastoma (MDB); Gastric cancer (GASC); Hepatocellular carcinoma (HCC)
  • 亚细胞定位:
    Cell junction, adherens junction. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle membrane. Cytoplasm. Cell membrane.
  • 蛋白家族:
    Adenomatous polyposis coli (APC) family
  • 组织特异性:
    Expressed in a variety of tissues: brain, small intestine, colon, thymus, skeletal muscle, heart, prostate, lung, spleen, ovary, testis kidney, placenta, blood and liver. Isoform 1A: Very strongly expressed in brain but has relatively low expression level
  • 数据库链接:

    HGNC: 583

    OMIM: 114550

    KEGG: hsa:324

    STRING: 9606.ENSP00000257430

    UniGene: Hs.158932