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Recombinant Rat Intestinal-type alkaline phosphatase 1 (Alpi) (Active)

In Stock
  • 货号:
    CSB-MP001627RA
  • 规格:
    ¥1908
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

产品详情

  • 纯度:
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Unit Definition:One unit is defined as the amount of enzyme required to cleave 1 nmol p-nitro-phenylphosphate(pNPP), in 1 minute at 37°C, pH10.0.The specific activity is >10370.37 U/mg.
  • 基因名:
    Alpi
  • Uniprot No.:
  • 别名:
    (IAP-1)(Intestinal alkaline phosphatase 1)(Intestinal alkaline phosphatase I)(IAP-I)
  • 分子结构:
  • 种属:
    Rattus norvegicus (Rat)
  • 蛋白长度:
    Full Length of Mature Protein
  • 来源:
    Mammalian cell
  • 分子量:
    55.9 kDa
  • 表达区域:
    21-511aa
  • 氨基酸序列
    VIPVEEENPVFWNQKAKEALDVAKKLQPIQTSAKNLILFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVPDSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRDWYSDADMPSSALQEGCKDIATQLISNMDIDVILGGGRKFMFPKGTPDPEYPGDSDQSGVRLDSRNLVEEWLAKYQGTRYVWNREQLMQASQDPAVTRLMGLFEPTEMKYDVNRNASADPSLAEMTEVAVRLLSRNPQGFYLFVEGGRIDQGHHAGTAYLALTEAVMFDSAIEKASQLTNEKDTLTLITADHSHVFAFGGYTLRGTSIFGLAPLNAQDGKSYTSILYGNGPGYVLNSGNRPNVTDAESGDVNYKQQAAVPLSSETHGGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEPYTDCGLAPPADENRPTTPVQN
  • 蛋白标签:
    N-terminal 10xHis-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述:

    Recombinant rat Alpi protein is an active protein produced in mammalian cells. Its expression region corresponds to the 21-511aa of the rat Alpi protein. It has high purity, >95% assessed by SDS-PAGE. The molecular weight of this Alpi protein on SDS-PAGE will appear at approximately 60-70kDa. Its endotoxin level is less than 1.0 EU/ug measured by the LAL method. It is expressed with a 10xHis-tag at N-terminus. The specific activity of this Alpi protein is >10370.37 U/mg.

    Alpi is the encoding gene for IAP-1, a brush border metalloenzyme that hydrolyzes phosphate from the lipid A moiety of LPS and thereby significantly reduces TLR-4 agonist activity. In addition to detoxifying LPS, IAP-1 is involved in dephosphorylating pro-inflammatory nucleotides like ATP and UDP, bicarbonate secretion regulation and duodenal pH maintenance, intestinal epithelial barrier protection by modulating the levels of tight junction proteins like occludins, claudins, and zonula occludens, gut microbial community modulation, and autophagy induction. IAP-1 deficiency has been associated with a variety of diseases, including inflammatory bowel disease (IBD), type 2 diabetes, ischemic heart disease, aging, and metabolic syndrome.

  • Datasheet & COA:
    Please contact us to get it.

产品评价

平均分:
5.0分 - 2 个评价

实验应用:结合实验/蛋白间互作

产品评价: 复购华美的蛋白啦,这次进行竞争ELISA 方法开发,结果显示蛋白有较好的结合活性,性质稳定,重复性好,各项参数指标达标,非常不错,将会一如既往的支持!

By 冯老师

实验应用:结合实验/蛋白间互作

产品评价: 我使用CSB-MP001627RA产品,进行竞争ELISA 方法开发,结果显示蛋白有较好的结合活性,性质稳定,重复性好,各项参数指标达标,本次属于再次购买,后续也将会继续支持该产品。

By 何老师

靶点详情

  • 功能:
    Alkaline phosphatase that can hydrolyze various phosphate compounds.
  • 亚细胞定位:
    Cell membrane; Lipid-anchor, GPI-anchor.
  • 蛋白家族:
    Alkaline phosphatase family
  • 数据库链接: