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  4. Recombinant Macaca mulatta Semaphorin-4D isoform 1 (SEMA4D), partial (Active)

Recombinant Macaca mulatta Semaphorin-4D isoform 1 (SEMA4D), partial (Active)

In Stock
  • 中文名称：
    猕猴SEMA4D重组蛋白
  • 货号：
    CSB-MP3947MOW
  • 规格：
    ￥1368
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC50 is 1.943-2.724 ng/mL. Biological Activity Assay
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque SEMA4D at 2 μg/mL can bind Anti-SEMA4D recombinant antibody (CSB-RA835707A1HU), the EC50 is 1.943-2.724 ng/mL.
  • 基因名：
    SEMA4D
  • Uniprot No.：
    H9FNS0
  • 别名：
    (EGK_07847)
  • 分子结构：
  • 种属：
    Macaca mulatta (Rhesus macaque)
  • 蛋白长度：
    Partial
  • 来源：
    Mammalian cell
  • 分子量：
    81.9 kDa
  • 表达区域：
    24-734aa
  • 氨基酸序列
    FAPIPRITWEHREVRLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSSLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSIAEEVFSHGKYMQSATVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGRRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALYQTESPSRGLVQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPVPTSTSCEPKIVINTVPQLHSEKTMYLKSSDNR
  • 蛋白标签：
    C-terminal 10xHis-tagged
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    SEMA4D Macaca mulatta recombinant protein produced in mammalian cells is a single polypeptide containing the amino acid sequence that encodes Phe24 - Arg734 of the Macaca mulatta SEMA4D and having a molecular weight of approximately 90 kDa on SDS-PAGE. This recombinant SEMA4D protein is expressed a 10xHis-tag at C-Terminus. Its purity is greater than 95% measured by SDS-PAGE. It contains low endotoxin, less than 1.0 EU/ug determined by the LAL method. In the functional ELISA, this SEMA4D protein can bind to the anti-SEMA4D recombinant antibody, with EC50 of 1.943-2.724 ng/mL, confirming it is an active protein.

    SEMA4D, also known as CD100, plays an important role in the immune and nervous systems. It is involved in platelet inactivation, angiogenesis stimulation, and bone formation regulation. SEMA4D expressed on osteoclasts is a coupling factor that suppresses osteoblast differentiation. As a result, inhibiting SEMA4D could be beneficial to bone growth. SEMA4D is also highly expressed in a variety of tumor tissues, including squamous cell carcinoma of the head and neck, prostate cancer, and colon cancer. SEMA4D-Plexin B1 signaling is frequently dysregulated in malignancies, implying that SEMA4D plays a role in cancer progression, invasion, tumor angiogenesis, tumor-associated macrophage modulation, and skeletal metastasis.

  • Datasheet & COA：
    Please contact us to get it.

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