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Recombinant Human Tumor necrosis factor ligand superfamily member 8 (TNFSF8), partial (Active)

In Stock
  • 货号:
    CSB-MP023996HU1c9
  • 规格:
    ¥1368
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized CD30(CSB-MP023983HU1) at 5 μg/ml can bind human CD30L, the EC50 is 4.169-6.360 ng/ml. Biological Activity Assay
  • 其他:

产品详情

  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized CD30(CSB-MP023983HU1) at 5 μg/ml can bind human CD30L, the EC50 is 4.169-6.360 ng/ml.
  • 基因名:
    TNFSF8
  • Uniprot No.:
  • 别名:
    TNFSF8; CD30L; CD30LG; Tumor necrosis factor ligand superfamily member 8; CD30 ligand; CD30-L; CD antigen CD153
  • 分子结构:
  • 种属:
    Homo sapiens (Human)
  • 蛋白长度:
    Partial
  • 来源:
    Mammalian cell
  • 分子量:
    45.8 kDa
  • 表达区域:
    63-234aa
  • 氨基酸序列
    QRTDSIPNSPDNVPLKGGNCSEDLLCILKRAPFKKSWAYLQVAKHLNKTKLSWNKDGILHGVRYQDGNLVIQFPGLYFIICQLQFLVQCPNNSVDLKLELLINKHIKKQALVTVCESGMQTKHVYQNLSQFLLDYLQVNTTISVNVDTFQYIDTSTFPLENVLSIFLYSNSD
  • 蛋白标签:
    N-terminal hFc-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    3-7 business days
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述:

    Human TNFSF8 amino acids (Gln63-Asp234) were expressed in the mammalian cells. The amino terminus contains the human IgG1 Fc-tag. The product is the partial length recombinant TNFSF8 protein. The SDS-PAGE assessed the purity of the protein to be greater than 94%. This TNFSF8 protein migrated to the molecular weight of about 55 kDa on the gel. The LAL method determined that the endotoxin content of the TNFSF8 protein was less than 1.0 EU/ug. This TNFSF8 protein has been validated to be biologically active through a functional ELISA. The receptor TNFRSF8 can bind to this TNFSF8 protein with the EC50 of about 4.169-6.360 ng/ml. It is in stock now.

    TNFSF8, also called CD153 or CD30L, is expressed on activated CD4+, CD8+ T cells, B cells, and macrophages. It mediates B cell growth and differentiation. Nancy D Marín and Luis F García found that TNFSF8-TNFRSF8 plays key roles in the T cell-dependent anti-mycobacterial immune response.

  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
  • 基因功能参考文献:
    1. circulant sCD30L is functionally active and that it may favor persistence of active inflammation by inducing apoptosis of CD30(+)T cells, known to down-modulate inflammation in rheumatoid synovitis. PMID: 24447865
    2. TNFSF8 is an important leprosy T1R susceptibility gene. PMID: 25320285
    3. The heritability of IgA levels is moderate and can partly be attributable to common variation in the CD30L locus. PMID: 24676358
    4. The TNFSF8 polymorphisms rs927374 and rs2295800 were associated with neutrophil count. This finding suggests that post-MI inflammatory response is genetically modulated. PMID: 22033252
    5. Positional candidate gene screening in the SPA2 locus allowed us to identify and replicate an association between a rare SNP located in TNFSF8 and spondylarthritis. PMID: 21480186
    6. a possible role of novel TNFSF8 variants in susceptibility to lung cancer. PMID: 21292647
    7. capability to up-regulate expression of CD30, release of soluble CD30 and production of IL-4 in pre-activated T cells upon co-culture PMID: 11728464
    8. Mast cells were found to be the predominant CD30 ligand-positive (CD30L-positive) cell in the chronic inflammatory skin diseases psoriasis and atopic dermatitis. PMID: 16964309
    9. CD153 antigen was expressed by synovial mast cells, and correlated with serum levels, in Rheumatoid Arthritis patients PMID: 19208589
    10. Single nucleotide polymorphism in TNFSF8 gene is associated with bone disease in myeloma. PMID: 19657367

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  • 亚细胞定位:
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族:
    Tumor necrosis factor family
  • 数据库链接:

    HGNC: 11938

    OMIM: 603875

    KEGG: hsa:944

    STRING: 9606.ENSP00000223795

    UniGene: Hs.494901