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Recombinant Human Tumor necrosis factor ligand superfamily member 18 (TNFSF18), partial (Active)

In Stock
  • 货号:
    CSB-MP891791HU
  • 规格:
    ¥1368
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 μg/ml can bind TNFSF18 (CSB-MP891791HU), the EC50 is 2.565 to 2.940 ng/ml. Biological Activity Assay
    • Activity
      Human TNFRSF18 protein hFc tag (CSB-MP896537HU) captured on COOH chip can bind Human TNFSF18 protein hFc and Flag tag (CSB-MP891791HU) with an affinity constant of 38.5 nM as detected by LSPR Assay. Biological Activity Assay
  • 其他:

产品详情

  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized TNFRSF18 at 2 μg/ml can bind TNFSF18 (CSB-MP891791HU), the EC50 is 2.565 to 2.940 ng/ml.②Human TNFRSF18 protein hFc tag (CSB-MP896537HU) captured on COOH chip can bind Human TNFSF18 protein hFc and Flag tag (CSB-MP891791HU) with an affinity constant of 38.5 nM as detected by LSPR Assay.
  • 基因名:
    TNFSF18
  • Uniprot No.:
  • 别名:
    (AITRL)(hGITRL)(AITRL)(GITRL)(TL6)
  • 分子结构:
  • 种属:
    Homo sapiens (Human)
  • 蛋白长度:
    Partial
  • 来源:
    Mammalian cell
  • 分子量:
    42.8 kDa
  • 表达区域:
    72-199aa
  • 氨基酸序列
    QLETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS
  • 蛋白标签:
    N-terminal hFc-Flag-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    3-7 business days
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述:

    The DNA fragment encoding the human TNFSF18 (Gln72-Ser199) was expressed with a C-terminal hFc-Flag-tag in the mammalian cells. The expressed protein is the recombinant human TNFSF18 protein with low endotoxin (< 1.0 EU/µg protein, LAL method) and high purity (> 94%, SDS-PAGE). This TNFSF18 protein displayed a molecular weight of approximately 45 kDa on the gel due to glycosylation. It is biologically active. In the functional ELISA, TNFRSF18 can bind to TNFSF18, with the EC50 is 2.565 to 2.940 ng/ml. In the LSPR assay, the human TNFRSF18 protein can bind to this TNFSF18 protein, with an affinity constant of 38.5 nM. This recombinant TNFSF18 protein is in stock now.

    TNFSF18, also called GITRL, is expressed in APCs and functions as a costimulatory molecule in the immune system. TNFSF18 engagement with its cognate receptor TNFRSF18 promotes proliferation and cytokine production of both CD4+ Teff and Treg cells, as well as drives the antitumor activity of CD8+ T cells. Besides, TNFRSF18/TNFSF18 signaling is implicated in the pathogenesis of inflammation, autoimmune diseases, and tumor.

  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation.
  • 基因功能参考文献:
    1. GITRL levels are significantly elevated in rheumatoid arthritis serum and synovial fluid and are positively correlated with autoantibody production in rheumatoid arthritis, suggesting a role of GITRL in the development of rheumatoid arthritis. PMID: 27098050
    2. GITRL modulates the activities of p38 MAPK and STAT3 to promote Th17 cell differentiation in autoimmune arthritis. PMID: 26657118
    3. An increase in GITRL may impair the balance of Th17/Treg, and contribute to the pathopoiesis of Hashimoto's thyroiditis. PMID: 25429429
    4. Serum GITRL levels were higher in SLE patients. PMID: 23251213
    5. Glucocorticoid-induced TNF-related ligand (GITRL) confers pseudoexpression to tumor cells by platelets, which results in GITRL expression by megakaryocytes and their platelet progeny. PMID: 22649191
    6. observation suggests a link between cytokine-regulated keratinocyte GITRL expression and its role in inflammatory responses in AD PMID: 22417213
    7. GITRL upregulation induced by IFN-beta on dendritic cells downregulates CTLA-4 on regulatory T (Treg) cells, facilitating proliferation of anergic Treg cells in multiple sclerosis treatment of multiple sclerosis patients. PMID: 22112394
    8. GITRL expression on Kupffer cells may mediate acute rejection in liver transplantation PMID: 21693309
    9. The incorporation of an isoleucine zipper motif could markedly improve the costimulation of hsGITRL. PMID: 20228835
    10. Upregulation by proinflammatory cytokines suggests GITRL may play important role in ocular immunity. High level of constitutive GITRL expression on photoreceptor inner segments suggests photoreceptors participate in regulation of ocular inflammation. PMID: 15326137
    11. Regulates ossteoclasst genersation and substantiate the major role played by the endothelium in bone physiology. PMID: 16179414
    12. Using a GITRL-transfected cell line, we demonstrate that GITRL promotes NK cell cytotoxicity and IFN-gamma production. PMID: 16397134
    13. GITRL could be a potential candidate for regulation of the ocular immune privilege and the balance between immune privilege and inflammation PMID: 16874737
    14. Constitutive expression of GITRL by tumor cells diminishes natural killer cell antitumor immunity. PMID: 17360848
    15. although huGITRL is not capable of alleviating Treg suppression of responder T cells, huGITRL overexpression on monocyte-derived DC enhances their capacity to induce antigen-specific T cell responses PMID: 17449724
    16. These observations raise the possibility that the GITRL-mediated inflammatory activation of macrophages is involved in the pathogenesis of inflammatory diseases. PMID: 17602748
    17. Levels of AITRL were significantly increased in serum of breast cancer patients PMID: 17914571
    18. hGITRL ectodomain displays considerable self-association/dissociation in solution with a dynamic equilibrium between trimeric and monomeric forms over the range of protein concentrations studied. PMID: 18040044
    19. identify multiple oligomeric species of hGITRL that possess distinct kinetics of ERK activation. PMID: 18378892
    20. The strong correlation of tumor incidence and elevated soluble GITRL levels indicates that soluble GITRL is released from cancers in vivo, leading to impaired NK cell immunosurveillance of tumors PMID: 18689545

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  • 亚细胞定位:
    Cell membrane; Single-pass type II membrane protein.
  • 蛋白家族:
    Tumor necrosis factor family
  • 组织特异性:
    Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.
  • 数据库链接:

    HGNC: 11932

    OMIM: 603898

    KEGG: hsa:8995

    STRING: 9606.ENSP00000385470

    UniGene: Hs.248197