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Recombinant Human Claudin-9 (CLDN9)-VLPs (Active)

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  • 货号:
    CSB-MP005511HU
  • 规格:
  • 图片:
    • CSB-MP005511HU is detected by Mouse anti-6*His monoclonal antibody.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/ml can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL. Biological Activity Assay
  • 其他:

产品详情

  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized Human CLDN9 at 10 μg/mL can bind Anti-CLDN6/9 recombinant antibody (CSB-RA005508MA1HU), the EC50 is 24.93-35.47 ng/mL.
  • 基因名:
    CLDN9
  • Uniprot No.:
  • 分子结构:
  • 种属:
    Homo sapiens (Human)
  • 蛋白长度:
    Full Length
  • 来源:
    Mammalian cell
  • 分子量:
    24.2 kDa
  • 表达区域:
    1-217aa
  • 氨基酸序列
    MASTGLELLGMTLAVLGWLGTLVSCALPLWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLLALLGLLVAITGAQCTTCVEDEGAKARIVLTAGVILLLAGILVLIPVCWTAHAIIQDFYNPLVAEALKRELGASLYLGWAAAALLMLGGGLLCCTCPPPQVERPRGPRLGYSIPSRSGASGLDKRDYV
  • 蛋白标签:
    C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述:

    This Human Claudin-9 (CLDN9) recombinant protein was produced in Mammalian cell, where the gene sequence encoding Human CLDN9 (1-217aa) was expressed with the C-terminal 10xHis tag. The activity was validated.
    In addition, this Human CLDN9 recombinant protein was developed through the Virus-Like Particles (VLPs) Platform. It is a four-pass transmembrane protein.

    CLDN9 (Claudin-9) is located on human chromosome 16p13.3, with a full length of 651 bases. The CLDN9 gene is overexpressed in the blood, pituitary and brain, and is highly expressed in endometrial cancer, liver cancer and gastric cancer cell lines. CLDN9 protein is mainly Localized at cell tight junctions, cell membranes and channel proteins.
    CLDN9 is a tight junction protein. Abnormal expression of CLDN9 will lead to impaired tight junctions function and reduced barrier function, resulting in increased tissue permeability, which will eventually lead to many diseases including hereditary, allergic diseases, infectious diseases of various systems and even tumors. Therefore, CLDN9 is regarded as a potential target for the treatment of cancer or other diseases.

  • Datasheet & COA:
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用:药物相关研究

产品评价: CLDN9被认为是治疗癌症或其他疾病的潜在靶点。CLDN9是一个4次跨膜的蛋白,表达全长难度很大。CUSA Bio通过其Vlp平台表达的重组人CLDN9蛋白,具有完整的生物活性,我使用CSB-MP005511HU产品及抗CLDN9抗体(CSB-RA005508MA1HU)进行ELISA实验,EC50为0.8032-2.566 ng/ml 。我司购买了CUSABIO的很多VLP产品,都是常规很难表达的,我们测试活性都很好,对我司的药物及研究贡献很大。确实是很有实力的一家公司!尤其是VLP产品很有特色!

By 翟老师

靶点详情

  • 功能:
    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.
  • 基因功能参考文献:
    1. CLDN9 may be an important biomarker for invasive pituitary oncocytomas. PMID: 25281028
    2. Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis. PMID: 19960275
    3. Mouse studies suggest that claudin-9 deficiency may cause hearing loss not only in mice but also in humans. PMID: 19696885
    4. Residues N38 and V45 in the first extracellular loop (EL1) of CLDN9 are necessary for HCV entry. PMID: 17804490
    5. CLDN9, clustered with CLDN6 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707

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  • 亚细胞定位:
    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族:
    Claudin family
  • 组织特异性:
    Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.
  • 数据库链接:

    HGNC: 2051

    OMIM: 615799

    KEGG: hsa:9080

    STRING: 9606.ENSP00000398017

    UniGene: Hs.296949