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Recombinant Human Cell adhesion molecule 1 (CADM1), partial (Active)

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  • 货号:
    CSB-MP004425HU
  • 规格:
    ¥1368
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2μg/mL can bind Human CRTAM (CSB-MP005998HU1),the EC50 is 17.82-21.71 ng/mL. Biological Activity Assay
  • 其他:

产品详情

  • 纯度:
    Greater than 95% as determined by SDS-PAGE.
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2 μg/mL can bind Human CRTAM (CSB-MP005998HU1), the EC50 is 17.82-21.71 ng/mL.
  • 基因名:
    CADM1
  • Uniprot No.:
  • 别名:
    Cell adhesion molecule 1 ; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Spermatogenic immunoglobulin superfamily; SgIgSF; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1
  • 种属:
    Homo sapiens (Human)
  • 蛋白长度:
    Partial
  • 来源:
    Mammalian cell
  • 分子量:
    38.5 kDa
  • 表达区域:
    45-374aa
  • 氨基酸序列
    QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDH
  • 蛋白标签:
    C-terminal 10xHis-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    3-7 business days
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node. Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells. Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. May contribute to the less invasive phenotypes of lepidic growth tumor cells.
  • 基因功能参考文献:
    1. miRNA-873 promoted Hepatocellular Carcinoma progression by targeting TSLC1. PMID: 29734164
    2. High CADM1 expression is associated with KSHV infection. PMID: 29698475
    3. the immunohistochemical expression of CADM1 has the potential to contribute as an auxiliary tool to the differential diagnosis between nevi and melanomas. PMID: 28843749
    4. These findings suggest that CADM1 expression is a novel prognostic factor for peripheral T cell lymphoma, not otherwise specified PMID: 28940014
    5. miR-21 enhances chemo-resistance in tongue cancer cells via directly targeting CADM1, and an inverse correlation between miR-21 and CADM1 expression in vivo. MiR-21 overexpression is attributed to MYCN-mediated transcriptional regulation, which is also predictive for a worse prognosis in tongue cancer PMID: 27055844
    6. We conclude that CADM1 was not associated with cell-cell interaction between lymphoma cells and macrophages, although CADM1 may be a useful marker of ATLL for diagnostic procedures. PMID: 28420814
    7. miR-21 overexpression promotes cardiac fibrosis via STAT3 signaling pathway by decrease CADM1 expression, indicating miR-21 as an important signaling molecule for cardiac fibrotic remodeling and atrial fibrillation. PMID: 28335740
    8. Pathway analysis of the genes associated with the 46 CpG sites revealed an enrichment of immune system process genes, including LYST (cg16962115, FDR = 1.24E-04), CADM1 (cg21933078, FDR = 1.22E-02) and NFATC1 (cg06784563, FDR = 1.46E-02) PMID: 28637314
    9. CADM1 mRNA expression was highly upregulated in normal tissues compared to esophageal squamous cell carcinoma tissues, indicating that the loss of CADM1 expression influenced the pathogenesis, invasion, and metastasis of ESCC. PMID: 28510248
    10. Eight and five of the nine samples were negative for cell adhesion molecule 1 and Osterix respectively. The other markers showed no statistical significance(CD151,ALP). osteoblastic differentiation can occur in carcinoma cells and that cell adhesion molecule 1 could be a useful marker for identifying this phenomenon in carcinoma tissues PMID: 28651491
    11. Our results suggest that, as in the CNS, CADM1 interactions drive exocytic site assembly and promote actin network formation. These results support the broader hypothesis that the effects of cell-cell contact on beta-cell maturation and function are mediated by the same extracellular protein interactions that drive the formation of the presynaptic exocytic machinery. These interactions may be therapeutic targets for re... PMID: 27072493
    12. High expression of CADM1 on most HTLV-1-infected cells in the face of enhanced cytotoxic T lymphocyte counterselection implies that CADM1 confers a strong benefit on the virus. PMID: 27105228
    13. Decreased expression of TSLC1 is associated with the differentiation in ovarian epithelial cancer. TSLC1 might be used as a molecular marker of severity in early stage ovarian cancer, and to help differentiating benign and malignant ovarian tumors. PMID: 27146728
    14. Suppression of tumorigenesis by CADM1 may be mediated by the Rb-E2F1 pathway. PMID: 27298105
    15. CADM1 prevents squamous cell carcinoma progression by reducing STAT3 activity PMID: 27035095
    16. Decreased expression of TSLC1 is correlated with the malignancy of non-muscle invasive bladder cancer tissues and low TSLC1 expression may serve as a predictor for bladder cancer recurrence and progression. PMID: 26894268
    17. CADM1 deletion is associated with neuroblastoma and ganglioneuroma. PMID: 26463893
    18. Hypermethylation of CADM1 gene may be highly associated with the development of cervical cancer. PMID: 26552048
    19. Our study showed that miR-126, by down-regulation CADM1, enhances migration and invasion in gastric cancer cells. PMID: 26464628
    20. This study revealed that Merkel cell polyomavirus -negative Merkel cell carcinomas significantly expressed higher CADM1 and lower MAL than Merkel cell polyomavirus -positive Merkel cell carcinomas PMID: 26772392
    21. CADM1/MAL methylation increases with severity of cervical intraepithelial neoplasia PMID: 26219541
    22. SynCAM1 delineates the postsynaptic perimeter of the synaptic cleft as determined by immunoelectron microscopy. PMID: 26687224
    23. increased CADM1 shedding appeared to contribute to the development of emphysema by generating alphaCTF and the ICD in lung epithelial cells. PMID: 26259600
    24. CADM1 shedding is increased in idiopathic interstitial pneumonia and contributes to alveolar epithelial cell apoptosis. PMID: 26231557
    25. Results show that CADM1 is downregulated in HPV-positive cervical cancers and that epigenetic alteration of CADM1 was more frequent in this type of cancer. The restoration of CADM1 expression may be a potential strategy for cervical cancer therapy. PMID: 25845528
    26. The use of CADM1, MAL, and MIR124-2 as biomarkers for full molecular screening in HIV infected women who are also positive for human papillomavirus. PMID: 26473640
    27. CADM1 induction was repressed in primary samples from AML patients at relapse. PMID: 25491945
    28. Our study reveals a novel mechanism of chronic NF-kappaB activation by CADM1 in HTLV-1 infected cells PMID: 25774694
    29. Trans-homophilic interaction of CADM1 activates PI3K by forming a complex with MAGuK-family proteins MPP3 and Dlg. PMID: 25268382
    30. In the absence of SynCAMs, selective axon-axon interactions are perturbed resulting in aberrant pathfinding of sensory axons. PMID: 25335893
    31. Increased ectodomain shedding of cell adhesion molecule 1 from pancreatic islets in type 2 diabetic pancreata. PMID: 24964098
    32. In a population-based association study, we confirm a role for CADM1 variants in increasing the risk of VT by interaction with protein C pathway abnormalities. PMID: 25306186
    33. The significant association of IGSF4 with HPV-positive head and neck squamous cell carcinoma suggests that HPV-positive tumors are to a greater extent driven by promoter hypermethylation in this tumor suppressor gene. PMID: 25569158
    34. HPV-positive oropharyngeal squamous cell carcinoma is associated with TIMP3 and CADM1 promoter hypermethylation. PMID: 25065733
    35. CADM1/TSLC1 is an antiinvasive gene, the overexpression of which inhibits the invasion of A375 cells. PMID: 25215547
    36. Cytology and bi-marker CADM1/MAL-methylation analysis perform complementary for CIN2+/CIN3+ detection when used as triage tool on cervical scrapes of HPV positive women. PMID: 25667975
    37. TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. PMID: 25031725
    38. The study showed that CADM1 is a direct target of miR-1246 leading to its down regulation in hepatocellular carcinoma cells. PMID: 25159494
    39. DNA methylation analysis of CADM1, MAL and miR124-2 in cervical scrapes consistently detects cervical cancer PMID: 25281766
    40. Loss of CADM1 expression is an independent prognostic factor especially associated with the development of brain metastases in breast cancer patients. PMID: 24833255
    41. We showed that the CADM1 versus CD7 plot is capable of discriminating clonally expanding HTLV-I-infected cells in indolent and acute-type T-cell leukemia-lymphomas and in asymptomatic carriers PMID: 24727323
    42. MPP3 and Dlg, membrane-associated guanylate kinase homologs (MAGuK) proteins, connect CADM1 with p85 of PI3K by forming a multi-protein complex at the periphery of cells. PMID: 24503895
    43. Data suggest that cell adhesion molecule 1 (CADM1) is a key adhesion receptor, which regulates mast cells (MCs) net adhesion. PMID: 24465823
    44. TGF-beta upregulates miR-182 expression to promote gallbladder cancer metastasis by targeting CADM1. PMID: 24445397
    45. CADM1 ectodomain shedding appeared to cause alveolar cell apoptosis in emphysematous lungs by producing alphaCTF that accumulated in mitochondria. PMID: 24092566
    46. The paper shows that CADM1 regulates mast cell net adhesion, both directly through CADM1-dependent adhesion, and indirectly through the regulation of other adhesion receptors. The latter is likely to occur via docking of KIT and polymerization of cortical F-actin. CADM1 is involved in docking of KIT to the cell surface and phosphotyrisine signaling, and polymerization of cortical F-actin. PMID: 24465823
    47. SynCAM, a novel putative tumor suppressor, suppresses growth and invasiveness of glioblastoma. PMID: 23907434
    48. Infection of NCI-H460, A549, and H1299 lung cancer cells with Ad.sp-E1A(Delta24)-TSLC1 induced apoptosis. PMID: 23503473
    49. Data suggest that tumor suppressor in lung cancer 1 (TSLC1) as tumor suppressor may play prominent roles in development and progression of cutaneous squamous cell carcinoma (CSCC) via regulation of different biological processes. PMID: 23812766
    50. The cell adhesion molecule Necl-2 competitively binds the immune receptor CRTAM. PMID: 23871486

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  • 亚细胞定位:
    Cell membrane; Single-pass type I membrane protein. Cell junction, synapse.
  • 蛋白家族:
    Nectin family
  • 数据库链接:

    HGNC: 5951

    OMIM: 605686

    KEGG: hsa:23705

    STRING: 9606.ENSP00000395359

    UniGene: Hs.370510