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  4. Recombinant Human C-C chemokine receptor type 8 (CCR8)-VLPs (Active)

Recombinant Human C-C chemokine receptor type 8 (CCR8)-VLPs (Active)

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  • 货号：
    CSB-MP004847HU
  • 规格：
    ￥5000
  • 图片：
    • CSB-MP004847HU is detected by Mouse anti-6*His monoclonal antibody.
    • CSB-MP004847HU is detected by Anti-CCR8 recombinant Antibody
    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized human CCR8 (CSB-MP004847HU) at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
    • The presence of VLP-like structures was confirmed by TEM
    • Loaded Anti-Human CCR8 Antibody (CSB-RA004847MA3HU) on 96-Flat plate, can bind Human CCR8, with an affinity constant of ＜1 pM as determined in BLI assay (Gator Prime).
    • The purity of VLPs was greater than 90% as determined by SEC-HPLC
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SEC-HPLC.
  • 内毒素：
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性：
    Measured by its binding ability in a functional ELISA. Immobilized human CCR8 (CSB-MP004847HU) at 5 μg/ml can bind Anti-CCR8 recombinant Antibody, the the EC50 is 11.13-17.29 ng/ml.
  • 基因名：
    CCR8
  • Uniprot No.：
    P51685
  • 别名：
    CCR8; CKRL1; CMKBR8; CMKBRL2; C-C chemokine receptor type 8; C-C CKR-8; CC-CKR-8; CCR-8; CC chemokine receptor CHEMR1; Chemokine receptor-like 1; CKR-L1; GPR-CY6; GPRCY6; TER1; CD antigen CDw198
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    Mammalian cell
  • 分子量：
    42.2 kDa
  • 表达区域：
    1-355aa
  • 氨基酸序列
    MDYTLDLSVTTVTDYYYPDIFSSPCDAELIQTNGKLLLAVFYCLLFVFSLLGNSLVILVLVVCKKLRSITDVYLLNLALSDLLFVFSFPFQTYYLLDQWVFGTVMCKVVSGFYYIGFYSSMFFITLMSVDRYLAVVHAVYALKVRTIRMGTTLCLAVWLTAIMATIPLLVFYQVASEDGVLQCYSFYNQQTLKWKIFTNFKMNILGLLIPFTIFMFCYIKILHQLKRCQNHNKTKAIRLVLIVVIASLLFWVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIYAFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSSVDYIL
  • 蛋白标签：
    C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
  • 产品提供形式：
    Liquid
    Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
  • 缓冲液：
    PBS, pH 7.4, 6% trehalose
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Recombinant full-length human CCR8 protein was expressed by the intact DNA sequence of the human CCR8 in mammalian cells. This recombinant CCR8 protein was fused with a 10xHis-tag at the C-terminus. It contains less than 1.0 EU/ug determined by the LAL method. The CCR8 protein is biologically active as it can react with the mouse anti-6xHis monoclonal antibody and anti-CCR8 recombinant antibody, respectively. Its VLP-like structure was observed and confirmed by TEM. This recombinant CCR8 protein is highly immunogenic because it carries viral protein.

    Past studies have shown that the CCR8 protein is mainly expressed on monocytes, Th2 cells, and Treg cells. Many basic studies have also proved CCR8 plays an essential role in the biological processes of lymph node migration of monocyte-derived dendritic cells and Th2 cell-mediated type 2 inflammation. Researchers have found that CCR8 is a good biomarker of Treg cells at tumor sites and a potential tumor immune target. Subsequent studies found that the presence or absence of CCR8 does not affect the migration ability of Treg and its function in the tumor microenvironment. The development of CCR8-targeted monoclonal antibody drugs is expected to specifically inhibit the function of Treg at the tumor site and contribute to cancer treatment.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

平均分:
5.0分 - 1 个评价

实验应用：药物相关研究

产品评价： CCR8多次跨膜，仅表达其胞外结构域会破坏其构象表位，造成部分抗原表位的丢失。华美生物公司表达全长蛋白，且具有较好的生物活性和免疫原性，对于我们后续开发抗体药物具有很大的帮助。赞赞赞

By 钱老师

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CCR8 Proteins

问答及客户评论

■ 常见问题解答
Q:

我注意到你提供了 CCR8 纳米圆盘，你能告诉我细胞内和细胞外结构域的暴露情况吗？ 我们想筛选一组能够检测该靶标的细胞内或细胞外表位的抗体； 对于 VLP，根据定义，细胞内结构域没有暴露，并不适合我的实验。

A:
VLP 只暴露胞外域 ，胞内域在包膜里面不暴露，主要就是筛识别胞外表位，所以VLP的可能不适合您的实验。 如果您想筛选一组能够检测该靶标的细胞内或细胞外表位的抗体，推荐：CSB-CF004847HU 是通过无细胞表达系统表达的蛋白全长，但是没有测活，不能保证识别内源样本，可以小规格订购尝试。或者您可以选择目的蛋白的胞内域或胞外域序列进行重组表达后，筛选识别靶标的细胞内或细胞外表位的抗体，但无法保证识别内源性样本。
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靶点详情

  • 功能：
    Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection.
  • 基因功能参考文献：
    1. Authors demonstrate the pivotal role of CCR8(+) Treg cells in restraining immunity and highlight the potential clinical implications of this discovery. PMID: 28533380
    2. Role of Conserved Disulfide Bridges and Aromatic Residues in Extracellular Loop 2 of Chemokine Receptor CCR8 for Chemokine and Small Molecule Binding. PMID: 27226537
    3. High CCR8 expression is associated with high recurrence in kidney cancer. PMID: 26716905
    4. findings suggest that CCR8 expression in ALCL is more closely related to the presence of DUSP22 rearrangements than to cutaneous involvement and that the function of CCR8 may extend beyond its skin-homing properties in this disease PMID: 25390351
    5. Epidermal-derived vitamin D3 metabolites and prostaglandins provide an essential cue for the localization of CCR8+ immune surveillance T cells within healthy human skin. PMID: 26002980
    6. CCL1-CCR8 interaction may play a critical role in lymphocytic recruitment in IgG4-related sclerosing cholangitis and type 1 autoimmune pancreatitis, leading to duct-centred inflammation and obliterative phlebitis. PMID: 23811304
    7. Identification of human CCR8 as a CCL18 receptor. PMID: 23999500
    8. CCR8(+) myeloid cell subset is expanded in patients with cancer. PMID: 23363815
    9. Data show that CCR8 expression by newly activated naive T cells is regulated by skin-specific factor(s) derived primarily from epidermal keratinocytes. PMID: 23043070
    10. C-terminal clipping of chemokine CCL1/I-309 enhances CCR8-mediated intracellular calcium release and anti-apoptotic activity PMID: 22479563
    11. The functional data from human macrophages suggest a potential cross talk between the CCR8 and the Toll-like receptor 4 (TLR4) pathways, both of which are present in chronic obstructive pulmonary disease patients. PMID: 21976223
    12. There may be a role for CCR8 in the recruitment of T cells to the lung in asthmatics. PMID: 20455898
    13. CCR8 mediates rescue from dexamethasone-induced apoptosis via an ERK-dependent pathway PMID: 12525579
    14. found in the central nervous system and is associated with phagocytic macrophages PMID: 12547701
    15. CCR8 genes and surrounding genomic regions these genes are the result of the duplication of an ancestral gene prior to the divergence of teleost fish. PMID: 12551893
    16. Transfected human CCR8-dependent activation of the RAS/MAPK pathway mediates anti-apoptotic activity of I-309/ CCL1 and vMIP-I. PMID: 12645948
    17. The induction of CCR8 under conditions associated with vascular smooth muscle cell proliferation and migration raises the possibility that CCR8 may play an important role in vessel wall pathology. PMID: 14576057
    18. the axis CCL1-CCR8 links adaptive and innate immune functions that play a role in the initiation and amplification of atopic skin inflammation PMID: 15814739
    19. CCR8 ligands are allotropic, binding to distinct sites within CCR8; the human immune system may have evolved to use CCL7 as a selective antagonist of viral chemokine activity at CCR8 but not those of the host ligand PMID: 17023422
    20. CCR8 is expressed by a small and heterogeneous population of peripheral blood CD4 memory T cells enriched in T helper type 2 (Th2) effector and T regulatory (Treg) cells. PMID: 17082609
    21. CCR8-expressing CD4-positive T lymphocytes are preferentially recruited from the periphery into the lungs of asthmatic individuals, driven by elevated CCL1 levels produced almost exclusively by mast cells and basophils. PMID: 17641040
    22. The combination of 17beta-E(2) with the environmental pollutant TCDD is involved in the pathogenesis of endometriosis via up-regulating the chemokine CCR8-I-309. PMID: 17693327

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 数据库链接：

    HGNC: 1609

    OMIM: 601834

    KEGG: hsa:1237

    STRING: 9606.ENSP00000326432

    UniGene: Hs.113222

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