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  4. CD63 Monoclonal Antibody

CD63 Monoclonal Antibody

文献引用 (1) 说明书
  • 中文名称：
    CD63鼠单克隆抗体
  • 货号：
    CSB-MA004950A0m
  • 规格：
    ￥1320
  • 图片：

    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate
      All lanes CD63 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 30-120 KD KDa
      Observed band size: 30-120 KD KDa
      Exposure time:1min

    • Western Blot
      Positive WB detected in: Raji whole cell lysate
      All lanes CD63 antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 30-120 KD KDa
      Observed band size: 30-120 KD KDa
      Exposure time:1min

    • IHC image of CSB-MA004950A0m diluted at 1:500 and staining in paraffin-embedded human glioma tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

    • IHC image of CSB-MA004950A0m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

    • IHC image of CSB-MA004950A0m diluted at 1:500 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.

    • Immunofluorescence staining of A549 cells with CSB-MA004950A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of Hela cells with CSB-MA004950A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Immunofluorescence staining of MCF-7 cells with CSB-MA004950A0m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

    • Overlay histogram showing A549 cells stained with CSB-MA004950A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

    • Overlay histogram showing Hela cells stained with CSB-MA004950A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

    • Overlay histogram showing HepG2 cells stained with CSB-MA004950A0m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

    • 1. Exosomes extracted from Hela cells
      2. Exosomes extracted from Hela cells
      3. Exosomes extracted from urine
  • 其他：

产品详情

  • 产品描述：
    CD63单克隆抗体（CUSABIO货号：CSB-MA004950A0m）是一款针对人源CD63抗原研发的高特异性科研试剂。CD63作为四次跨膜蛋白超家族成员，广泛表达于血小板、溶酶体及外泌体膜表面，参与细胞间信号传导、膜运输调控等重要生物学过程，尤其在肿瘤微环境调控、免疫细胞活化及外泌体生物标志物研究中具有重要价值。该抗体经严格验证可兼容ELISA、Western Blot、免疫组化、免疫荧光及流式细胞术五大实验平台，满足多维度科研需求。在肿瘤生物学领域可用于黑色素瘤、乳腺癌等实体瘤相关研究；在免疫学方向支持T细胞活化机制探索；在外泌体研究中可作为特异性膜标记物进行囊泡分离与追踪。其优异的人源特异性使其成为研究血小板活化机制、溶酶体相关膜蛋白运输以及细胞粘附迁移过程的理想工具，适用于细胞培养模型、组织切片样本及原代细胞分析等实验体系，为探索肿瘤转移、免疫调控及细胞通讯机制提供可靠支持。
  • 产品名称：
    Mouse anti-Homo sapiens (Human) CD63 Monoclonal Antibody antibody
  • Uniprot No.：
    P08962
  • 基因名：
    CD63
  • 别名：
    CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Lysosome integral membrane protein 1; Limp1; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD antigen CD63
  • 宿主：
    Mouse
  • 反应种属：
    Human
  • 免疫原：
    Recombinant Human CD63 antigen protein (103-203AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    1H1E11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB WB：1:1000-1：8000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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靶点详情

  • 功能：
    Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
  • 基因功能参考文献：
    1. Signaling focal contacts via CD63 were identified to facilitate cell adhesion, migration and mediate extracellular matrix-physical cues to modulate hematopoietic stem cells and progenitor cells function. PMID: 28566689
    2. Human CD63-GFP expression was controlled under the rat Sox2 promoter (Sox2/human CD63-GFP), and it was expressed in undifferentiated fetal brains. PMID: 29208635
    3. our results identify the CD63-syntenin-1-ALIX complex as a key regulatory component in post-endocytic HPV trafficking. PMID: 27578500
    4. CD63 and exosome expression is altered in scleroderma dermal fibroblasts PMID: 27443953
    5. CD63 is a critical player in LMP1 exosomal trafficking and LMP1-mediated enhancement of exosome production and may play further roles in limiting downstream LMP1 signaling. PMID: 27974566
    6. TIMP1 signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. PMID: 27506299
    7. Concentrations of Cd63 were elevated in gingival crevicular fluid of patients with pre-eclampsia. PMID: 26988336
    8. CD163(+) TAMs in oral premalignant lesions coexpress CD163 and STAT1, suggesting that the TAMs in oral premalignant lesions possess an M1 phenotype in a Th1-dominated micromilieu. PMID: 26242181
    9. Our findings reveal that elevated levels of TIMP-1 impact on neutrophil homeostasis via signaling through CD63. PMID: 26001794
    10. These findings indicate that rhTIMP-1 promotes clonogenic expansion and survival in human progenitors via the activation of the CD63/PI3K/pAkt signaling pathway PMID: 26213230
    11. It was shown that treatment of macrophages with anti-CD63 inhibits CCR5-mediated virus infection in a cell type-specific manner, but that no inhibition of CXCR4-tropic viruses occurs. PMID: 25658293
    12. TM4SF5 interacted with CD151, and caused the internalization of CD63 from the cell surface. PMID: 25033048
    13. Diesel exhaust exposure during exercise induces platelet activation as illustrated by a dose-response increase in the release of CD62P and CD63. PMID: 25297946
    14. Data show that CD63 is a crucial player in the regulation of the tumor cell-intrinsic metastatic potential by affecting cell plasticity. PMID: 25354204
    15. CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells. PMID: 24940653
    16. these results indicated that high glycosylation of CD63 by RPN2 is implicated in clinical outcomes in breast cancer patients PMID: 24884960
    17. Collectively, these findings indicate that CD63 may support Env-mediated fusion as well as a late (post-integration) step in the HIV-1 replication cycle. PMID: 24507450
    18. Data suggest that TIMP1 (tissue inhibitor of metalloproteinase-1) acts as chemoattractant for neural stem cells (NSC); TIMP1 enhances NSC adhesion, migration, and cytoskeletal reorganization; these effects are dependent on CD63/CD29 (integrin beta1). PMID: 24635319
    19. Timp1 is assembled in a supramolecular complex containing CD63 and beta1-integrins along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway. PMID: 23522389
    20. Antigen-induced p38 MAPK phosphorylation in human basophils essentially contributes to CD63 upregulation PMID: 18727065
    21. Loss of CD63 has a similar phenotype to loss of P-selectin itself, thus CD63 is an essential cofactor to P-selectin. PMID: 21803846
    22. shRNA knockdown in B lymphoblastoid cell line results to increased CD4(+) T-cell recognition PMID: 21660937
    23. decreased levels of CD63 were associated with distant and lymph node metastasis status and does play a direct role in human gastric carcinogenesis. PMID: 21521534
    24. C-terminal modifications that retain LMP1 in Golgi compartments preclude assembly within CD63-enriched domains and/or exosomal discharge leading to NF-kappaB overstimulation. PMID: 21527913
    25. These findings suggest that CD63 plays an early post-entry role prior to or at the reverse transcription step. PMID: 21315401
    26. ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin beta1 PMID: 21149578
    27. Surface expression of the novel CD63 variant is a distinguishing feature of mast cells, which are are stable, multiple-use cells capable of surviving and delivering several consecutive hits PMID: 20337613
    28. this work provides the first evidence of a TIMP-4/CD63 association in astrocytoma tumor cells PMID: 20693981
    29. CD63 expression results from only the anaphylactic degranulation form of histamine release. PMID: 20633031
    30. Serum sCD163 is a homogenous protein covering more than 94% of the CD163 ectodomain including the haptoglobin-hemoglobin -binding region. PMID: 19581020
    31. Results show that AP-3 is absolutely required for the delivery of CD63 to lysosomes via the trans-Golgi network. PMID: 11907283
    32. downregulation of CD63 antigen is associated with breast tumor progression PMID: 12579280
    33. possible role in HIV-1 infections specific for macrophages PMID: 12610138
    34. Post-translational modification of CD63 may be involved in the functional and morphological changes of MHC class II compartments that occur during dendritic cell maturation PMID: 12755696
    35. relationships between the expression levels of CD61, CD63, and PAC-1 on the platelet surface and the incidences of acute rejection and tubular necrosis as well as the recovery of graft function after renal transplantation PMID: 12826159
    36. Upon platelet interaction with fibrinogen, cholesterol accumulated at the tips of filopodia and at the leading edge of spreading cells; cholesterol-rich raft aggregation was accompanied by concentration of c-Src and CD63 in these cell domains PMID: 12871315
    37. The study on CD63 included its chemistry eg. if it had and O-linked carbohydrate that was digested with O-glycanase. PMID: 12974720
    38. CD63 serves as an adaptor protein that links its interaction partners to the endocytic machinery of the cell. PMID: 14660791
    39. results suggest that CD9, CD63, CD81, and CD82 could play a role in modulating the interactions between immature DCs and their environment, slowing their migratory ability. However, only CD63 would intervene in the internalization of complex antigens PMID: 15130945
    40. Constitutive expression of CD63 may indicate that this factor does not play a direct role in thyroid carcinogenesis PMID: 15375577
    41. CD63 represents an activation-induced reinforcing element, whose triggering promotes sustained and efficient T cell activation and expansion. PMID: 15528334
    42. The linkage of CD63 with PI 4-kinase may result in the recruitment of this signaling enzyme to specific membrane locations in the platelet where it influences phosphoinositide-dependent signaling and platelet spreading. PMID: 15711748
    43. This study identifies a trafficking pathway from CD63-positive multivesicular bodies to the bacterial inclusion, a novel interaction that provides essential lipids necessary for maintenance of a productive intracellular infection. PMID: 16410552
    44. CD63 is recruited to already-budded Weibel-Palade bodies by an AP-3-dependent route PMID: 16683915
    45. CD63-syntenin-1 complex is abundant on the plasma membrane PMID: 16908530
    46. CD63 is a cell surface binding partner for TIMP-1, regulating cell survival and polarization via TIMP-1 modulation of tetraspanin/integrin signaling complex. PMID: 16917503
    47. Chronic urticaria serum-induced CD63 expression assay performed on atopic whole blood by means of tricolor flow cytometry could be the most useful tool for identification of a subset of patients with autoimmune chronic urticaria. PMID: 16918509
    48. In conclusion, using well-defined experimental conditions, the measurement of CD203c up-regulation on basophils in response to specific allergens is as reliable as CD63-BAT for the in vitro diagnosis of patients with IgE-mediated allergy. PMID: 17275019
    49. positive correlation between CD63 and erythrocyte sedimentation rate in rheumatoid arthritis PMID: 17279322
    50. Results suggest that CD63 can be a biomarker for predicting the prognosis in early stages of lung adenocarcinoma. PMID: 17350713

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Secreted, extracellular exosome. Cell surface.
  • 蛋白家族：
    Tetraspanin (TM4SF) family
  • 组织特异性：
    Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • 数据库链接：

    HGNC: 1692

    OMIM: 155740

    KEGG: hsa:967

    STRING: 9606.ENSP00000257857

    UniGene: Hs.445570

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