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Rat Prostaglandin E2,PG-E2 ELISA Kit

  • 中文名称:
    大鼠前列腺素E2(PGE2)酶联免疫试剂盒
  • 货号:
    CSB-E07967r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    大鼠前列腺素E2(PGE2)酶联免疫试剂盒(CSB-E07967r)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的PG-E2含量。PG-E2即前列腺素E2,它在体内广泛存在,参与多种生理和病理过程。在炎症、免疫调节、肿瘤发生发展等方面扮演重要角色。研究机制主要围绕其与受体结合,激活多条信号通路,影响细胞增殖、分化和炎症因子释放等过程。试剂盒检测范围为0.5 pg/mL-100 pg/mL,适用于体外实验研究,例如探究炎症模型中PGE2的动态变化、评估药物或化合物对前列腺素代谢通路的影响,以及组织微环境中脂质介质的定量分析等科研场景,为炎症机制研究、药效学评价及生物标志物探索提供高效检测工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    PG-E2
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    0.5 pg/mL-100 pg/mL
  • 灵敏度:
    0.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 91-99  
    1:2 Average % 92  
    Range % 87-96  
    1:4 Average % 93  
    Range % 89-98  
    1:8 Average % 105  
    Range % 99-109  
  • 回收率:
    The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-99  
    EDTA plasma (n=4) 101 96-104  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    100 0.228 0.224 0.226    
    25 0.365 0.348 0.357    
    8 0.560 0.539 0.550    
    2 0.813 0.787 0.800    
    0.5 1.258 1.234 1.246    
    0 2.031 2.176 2.104    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型:血清

样品信息:大鼠

稀释比:没有稀释

产品评价: 检测pg-e2在血清中的含量,该试剂盒操作较方便,已经备好六只标准品,自行配液误差较小,从实验结果可以看出效果较好,希望其他试剂盒也能配好标准品,尽量减少操作可能出现误差,简化流程

By 李老师

靶点详情

  • 最新研究进展:
    PGE2是一种前列腺素,与炎症和癌症等多种疾病有关。近期的研究表明,PGE2可以在体内诱导肝癌干细胞分化为肝癌细胞,从而促进肝癌的发生和发展。此外,研究人员还发现,通过靶向PGE2的合成酶和受体可以减少肿瘤的生长和转移,从而为肿瘤治疗提供新的策略。