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  4. Rat Interleukin 4,IL-4 ELISA KIT

Rat Interleukin 4,IL-4 ELISA KIT

文献引用 (38) 说明书下载
  • 中文名称：
    大鼠白介素4(IL-4)酶联免疫试剂盒
  • 货号：
    CSB-E04635r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠白介素4(IL-4)酶联免疫试剂盒（CSB-E04635r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL4含量。IL-4是一种由CD4+T细胞产生的细胞因子，主要参与Th2型免疫反应，诱导B细胞发育和M2型巨噬细胞分化。其研究机制涉及IL-4与IL-4R结合，激活JAK激酶和STAT6，进而调节多种免疫相关基因的表达。IL-4在过敏性疾病、肿瘤和免疫调节中发挥重要作用。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于科研领域中对炎症性疾病模型、免疫调控机制或药物干预效果的评估；为研究者探索免疫相关疾病的分子机制、疫苗开发中的 Th2 型免疫应答分析，以及环境因素对免疫系统影响的实验研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il4 ELISA Kit; Il-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell IGG differentiation factor ELISA Kit; B-cell growth factor 1 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
  • 缩写：
    IL4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    101

    Range %

    95-110

    1:2

    Average %

    97

    Range %

    91-105

    1:4

    Average %

    99

    Range %

    94-104

    1:8

    Average %

    93

    Range %

    89-98

  • 回收率：

    The recovery of human LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    90-101

    EDTA plasma (n=4)

    98

    94-101

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    1000

    3.065

    2.834

    2.949

    2.794

    500

    2.708

    2.567

    2.637

    2.482

    250

    2.135

    2.028

    2.082

    1.926

    125

    1.434

    1.429

    1.431

    1.276

    62.5

    0.774

    0.721

    0.748

    0.592

    31.2

    0.468

    0.453

    0.461

    0.305

    15.6

    0.307

    0.294

    0.300

    0.145

    0

    0.156

    0.155

    0.155

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Il4 Proteins

靶点详情

  • 最新研究进展：
    IL-4是一种细胞因子，它在机体免疫反应中发挥重要作用，特别是在过敏和响应寄生虫感染时。近年来的研究表明，IL-4在多种疾病的发生和发展中也具有潜在的作用。例如，在肿瘤中，IL-4能够刺激免疫细胞对肿瘤细胞的杀伤作用，因此被视为一种潜在的肿瘤免疫治疗药物。此外，IL-4还被发现在许多自身免疫性疾病中表达水平升高，如类风湿关节炎、多发性硬化症等，因此也成为这些疾病治疗的研究热点之一。
  • 功能：
    Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
  • 基因功能参考文献：
    1. age-related decrease in IL-4 expression exacerbated surgery-induced cognitive deficits and exaggerated the neuroinflammatory responses PMID: 28414034
    2. IL-4 Release from a Biomimetic Scaffold for the Temporally Controlled Modulation of Macrophage Response PMID: 26951461
    3. Formoterol synergizes with des-ciclesonide to inhibit IL-4 expression in IgE/antigen-induced mast cells by inhibiting JNK activation. PMID: 26003274
    4. CaSR activation promoted T-cell apoptosis and the secretion of pro-inflammatory cytokine TNF-alpha and anti-inflammatory cytokines IL-4. PMID: 25256599
    5. IL-4 was found to augment the mRNA expression of ICAM-1. PMID: 23904363
    6. IL-4 has a modulatory effect on the enzymatic compartment of the antioxidant defense system in the brain of rats. PMID: 24319732
    7. IL-4 promoted the activation of effector cells to mediate rejection and did not support alloantigen-specific Treg that could transfer specific tolerance. PMID: 24139939
    8. The level of IL-4 is elevated in masseter muscle of both sexes following chemically induced inflammation. PMID: 20969918
    9. Treatment with intranasal interferon gamma can inhibit production of IL4 and IL5 in allergic rhinitis. PMID: 18510219
    10. IL4 in bronchoalveolar lavage fluid of allergic rhinitis rats was elevated. PMID: 16229173
    11. endogenous expression of antiinflammatory cytokine IL-4 regulates the extent of acute macrophage activation and confines the ensuing secondary cavity formation after spinal cord trauma PMID: 20623539
    12. passive rats exposed to emotional stress were characterized by a decreased blood levels of TNF alpha and IL-4 as compared to behaviorally passive controls and behaviorally active animals exposed to stress. PMID: 20120734
    13. A single treatment with IL-4 via retrovirally transduced lymphocytes partially protects against type 1 diabetes in BioBreeding rats. PMID: 12004164
    14. Gene transfer of IL-4 may represent a potential therapeutic approach to induce tolerance to splenic allografts. PMID: 15251393
    15. IL-4 appears to be an important Th2 cytokine involved in the induction of the L-arginine/iNOS pathway in eosinophils PMID: 15826818
    16. synthesis and release from mast cells mediated by Mycoplasma pneumoniae P1 adhesin PMID: 16169702
    17. HSV-mediated expression of IL-4 effectively reduces the behavioral manifestations of neuropathic pain, and reverses some of the biochemical and histologic correlates of neuropathic pain at the spinal level. PMID: 16503976
    18. Data show that islets of non-diabetic BB rats become positive to both IL-1beta and IL-4 very early on, and that production of these interleukins together with the expression of CD4 and CD25 are predictive for type 1 diabetes onset in non-diabetic BB rats. PMID: 16972262
    19. These data suggest that IL-4 may provide a significant immunomodulatory signal which can protect against microglia-mediated neurotoxicity by suppressing the production and release of free radicals. PMID: 17018025
    20. We conclude that IL-4 exerts its anti-inflammatory effects in a limited and target-specific manner, which is delicately regulated by the cellular microenvironment. T PMID: 17442043
    21. Our data demonstrate, for the first time, the neuroprotective effect of IL-4 through cAMP/PKA pathway in thapsigargin-induced photoreceptor cell death. PMID: 19235892

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-4/IL-13 family
  • 数据库链接：

    KEGG: rno:287287

    STRING: 10116.ENSRNOP00000010029

    UniGene: Rn.108255

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