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  4. Rat Interleukin 3,IL-3 ELISA KIT

Rat Interleukin 3,IL-3 ELISA KIT

说明书下载
  • 中文名称：
    大鼠白介素3(IL-3)酶联免疫试剂盒
  • 货号：
    CSB-E07436r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠白介素3(IL-3)酶联免疫试剂盒（CSB-E07436r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL3含量。IL3，即白细胞介素-3，是一种由激活的T细胞和其他细胞产生的细胞因子，主要在造血过程中发挥作用，调控粒细胞和单核细胞-巨噬细胞的产生、分化和功能。其研究机制涉及IL3如何通过激活细胞内信号通路，如Ras-Raf-ERK和JAK/STAT，来促进造血细胞的生长和分化。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域中对IL-3表达水平的研究，例如在造血系统疾病模型、感染性疾病动物实验或免疫相关病理机制探讨中，通过检测血液或组织样本中的IL-3浓度变化，揭示其在炎症反应、细胞分化及免疫调控中的作用规律。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
  • 缩写：
    IL3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %100
    Range %95-113
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %92-104
  • 回收率：
    The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9387-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.436 2.276 2.356 2.214
    1001.975 2.014 1.995 1.853
    501.435 1.372 1.404 1.262
    250.868 0.913 0.891 0.749
    12.50.553 0.519 0.536 0.394
    6.250.387 0.407 0.397 0.255
    3.120.285 0.276 0.281 0.139
    00.144 0.140 0.142
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

相关产品

Il3 Proteins

靶点详情

  • 功能：
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-3 family
  • 组织特异性：
    Activated T-cells, mast cells, natural killer cells.
  • 数据库链接：

    UniGene: Rn.10652

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