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  4. Rat Complement fragment 5,C5 ELISA Kit

Rat Complement fragment 5,C5 ELISA Kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠补体片断5(C5)酶联免疫试剂盒
  • 货号：
    CSB-E14363r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：
    大鼠补体片断5(C5)酶联免疫试剂盒（CSB-E14363r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的C5含量。C5是补体系统中的关键成分，在免疫防御中起重要作用。异常激活会引发多种疾病。研究机制主要围绕抑制其激活，以减少炎症反应和组织损伤。目前针对C5的治疗手段，如单抗药物，在多种病症中展现出良好应用前景。试剂盒检测范围为9.38 ng/mL-600 ng/mL，可应用于大鼠模型中的补体系统功能研究，例如评估炎症性疾病动物模型的病理进程、药物干预对补体激活的影响，或探究组织损伤修复过程中C5的动态变化，为免疫学、药理学及基础医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    C5Complement C5 [Cleaved into: C5a anaphylatoxin] ELISA kit; Fragment ELISA kit
  • 缩写：
    C5
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    9.38 ng/mL-600 ng/mL
  • 灵敏度：
    2.34 ng/mL
  • 反应时间：
    1-5h
  • 上样量：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat C5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:200

    Average %

    104

    Range %

    97-111

    1:400

    Average %

    101

    Range %

    94-108

    1:800

    Average %

    98

    Range %

    93-103

    1:1600

    Average %

    91

    Range %

    84-97

  • 回收率：

    The recovery of rat C5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    104

    99-110

    EDTA plasma (n=4)

    95

    88-101

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    600

    2.218

    2.134

    2.176

    2.070

    300

    1.864

    1.767

    1.815

    1.710

    150

    1.346

    1.298

    1.322

    1.216

    75

    0.937

    0.929

    0.933

    0.827

    37.5

    0.601

    0.611

    0.606

    0.500

    18.75

    0.436

    0.453

    0.445

    0.339

    9.38

    0.302

    0.294

    0.298

    0.192

    0

    0.106

    0.105

    0.106

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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C5 Proteins

靶点详情

  • 功能：
    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.; Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
  • 亚细胞定位：
    Secreted.
  • 数据库链接：

    STRING: 10116.ENSRNOP00000025534

    UniGene: Rn.21259

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