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中文名称:大鼠C反应蛋白(CRP)酶联免疫试剂盒
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货号:CSB-E07922r
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规格:96T/48T
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价格:¥3900/¥2500
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其他:
产品详情
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产品描述:CRP,即C-反应蛋白(C-reactive protein),是一种急性相蛋白,主要由肝脏合成,可作为炎症的非特异性生物标志物。当机体遇到感染、损伤、炎症等刺激时,肝脏合成和分泌CRP,其浓度会在数小时内显著升高。因此,CRP常被用于评估感染、炎症和自身免疫性疾病等疾病的活动程度和预后,也可用于评估心血管疾病和癌症等疾病的风险。正常情况下,血浆中CRP的浓度很低,但在炎症反应期可升高数千倍。华美生物所提供的Rat C-Reactive Protein,CRP ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、细胞培养上清、组织匀浆、细胞裂解液样本中的CRP,其灵敏度为7.81 ng/mL,检测范围为31.25 ng/mL-2000 ng/mL。
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别名:Crp ELISA kit; Ptx1C-reactive protein ELISA kit
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缩写:
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, cell culture supernates, tissue homogenates, cell lysates
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检测范围:31.25 ng/mL-2000 ng/mL
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灵敏度:7.81 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 89 Range % 85-96 1:4 Average % 93 Range % 89-97 1:8 Average % 99 Range % 92-105 -
回收率:
The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 88-97 EDTA plasma (n=4) 96 91-99 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 2000 2.664 2.701 2.683 2.541 1000 1.701 1.716 1.709 1.567 500 1.173 1.182 1.178 1.036 250 0.604 0.589 0.597 0.455 125 0.398 0.372 0.385 0.243 62.5 0.302 0.288 0.295 0.153 31.25 0.234 0.227 0.231 0.089 0 0.144 0.140 0.142 -
数据处理:
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货期:3-5 working days
引用文献
- L-theanine regulates the immune function of SD rats fed high-protein diets through the FABP5/IL-6/STAT3/PPARα pathway Y Zhang,Food and chemical toxicology,2023
- Investigating the therapeutic potential of sinomenine in rheumatoid arthritis: anti-inflammatory, antioxidant, and immunomodulatory mechanisms J Li,Naunyn-Schmiedeberg's archives of pharmacology,2023
- Co-spray-dried poly-L-lysine with L-leucine as dry powder inhalations for the treatment of pulmonary infection: Moisture-resistance and desirable aerosolization performance X Zhang,International journal of pharmaceutics,2023
- Rosuvastatin and diosmetin inhibited the HSP70/TLR4 /NF-κB p65/NLRP3 signaling pathways and switched macrophage to M2 phenotype in a rat model of acute kidney injury induced by cisplatin F Feng,Biomedicine & pharmacotherapy,2024
- Therapeutic effects of combining curcumin and swimming in osteoarthritis using a rat model MM Saber,Biomedicine & pharmacotherapy,2023
- Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis G Angelini,Nature communications,2022
- Rosuvastatin and low dose carvedilol combination protects against isoprenaline- induced myocardial infarction in rats: Role of PI3K/Akt/Nrf2/HO-1 signaling SA Baraka,Clinical and Experimental Pharmacology and Physiology,2021
- Diabetogenic diet-induced insulin resistance associates with lipid droplet proteins and adipose tissue secretome, but not with sexual dimorphic adipose tissue fat accumulation in wistar rats SM Jeyakumar,Biochem Biophys Rep,2020
- Anti-inflammatory and antidiabetic effects of grape-derived stilbene concentrate in the experimental metabolic syndrome A Kubyshkin,Journal of Diabetes & Metabolic Disorders,2020
- The gut microbiota may be a novel pathogenic mechanism in loosening of orthopedic implants in rats MM Moran,FASEB J,2020
- Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and interscapular brown adipose tissue of obese rats Q Cao,FASEB J,2020
- Lentiviral gene therapy vectors encoding VIP suppressed diabetes-related inflammation and augmented pancreatic beta-cell proliferation F Erendor,Gene Therapy,2020
- Modulation of the Liver Protein Carbonylome by the Combined Effect of Marine Omega-3 PUFAs and Grape Polyphenols Supplementation in Rats Fed an Obesogenic … Luc铆a M茅ndez,marine drugs,2019
- Diallyl sulfide alleviates cyclophosphamide-induced nephropathic encephalopathy in rats Galal S M, et al,Toxicology Mechanisms and Methods,2019
- The Effect of Endurance Training and Purslane Seed Consumption on NF-κB and CRP in the Heart Tissue of Rats Exposed to Oxidative Damage Induced by H2O2 Valiollah Shahedi,Journal of Archives In Military Medicine,2019
- Chronic paradoxical sleep deprivation-induced depression Ma W, et al,Life Sciences,2019
- Salvianolate reduces atrial fibrillation through suppressing atrial interstitial fibrosis by inhibiting TGF-β1/Smad2/3 and TXNIP/NLRP3 inflammasome signaling pathways in post-MI rats HuiliangQiu.et al,Phytomedicine,2018
- Chios mastic gum decreases renin levels and ameliorates vascular remodeling in renovascular hypertensive rats Aspasia I.Tzani.et al,Biomedicine & Pharmacotherapy,2018
- Effects of Fine Particulate Matter (PM2. 5) on Erectile Function and Its Potential Mechanism in Rats. Wang X.et al,Urology,2016
- PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY Hakan Guzel.et al,International Surgery,2016
- Cardioprotective Effects of HuoxueAnshen Recipe against Myocardial Injuries Induced by Sleep Deprivation in Rats Rong Yuan.et al,Evidence-Based Complementary and Alternative Medicine,2017
- Dietary strawberry seed oil affects metabolite formation in the distal intestine and ameliorates lipid metabolism in rats fed an obesogenic diet. Jurgo?ski A.et al, Food & nutrition research,2015
- The synergistic effect of antiglycating agents (MB-92) on inhibition of protein glycation, misfolding and diabetic complications in diabetic-atherosclerotic rat S. Mahdavifard. et al,European Journal of Medicinal Chemistry,2015
- Age protects from harmful effects produced by chronic intermittent hypoxia Quintero M. et al,J Physiol,2016
- SP600125 promotes resolution of allergic airway inflammation via TLR9 in an OVA-induced murine acute asthma model Wu HM. et al,Mol Immunol. ,2015
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Frisch J. et al,Tissue Eng Part A. ,2015
- Dietary Supplementation with Raspberry Seed Oil Modulates Liver Functions, Inflammatory State, and Lipid Metabolism in Rats Fotschki B. et al,J Nutr,2015
- Parthenolide reduces empty lacunae and osteoclastic bone surface resorption induced by polyethylene particles in a murine calvarial model of peri??mplant osteolysis Zawawi MS et al,J Biomed Mater Res A,2015
- Expression of myostatin in early postnatal mouse masseter and rectus femoris muscles Takada H1, Miwa Y1, Sato I2,Histol Histopathol,2015
- Effects of Neurolytic Celiac Plexus Block on Liver Regeneration in Rats with Partial Hepatectomy Li J et al,PLoS One,2013
- Effects of ozone and fine particulate matter (PM2.5) on rat system inflammation and cardiac function Wang G et al,Toxicol Lett,2012
问答及客户评论
■ 客户评论
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Sample type: Supernatant
Sample species: Rat
Review: Serum concentrations of biomarkers, including TGF-β1, TNFα, IL-1β, IL-6, IL-18, CRP, and BNP were measured by Elisa kits following manufacture instructions. As shown in figure, the levels of TGF-β1, CRP, IL-6 and BNP were significantly increased in MI rats than Sham. Salvianolate treatment dose-dependently reduced these levels compared with MI rats.
PMID: 30466624 -
Sample type: Serum
Sample species: Rat
Review: Renin, ANGII, C-reactive protein (CRP) and interleukin-6 (IL-6) levels were determined by ELISA. Our results advocate a positive correlation between CRP serum levels and CSA, which is indicative for vascular hypertrophy . Irrespective of the induction of hypertension, CRP may be implicated as mediator of vascular remodeling after injury
PMID: 30021383 -
Sample type: Serum
Sample species: Rat
Review: Frozen aliquots of serum samples were thawed firstly on ice. Subsequently, commercial ELISA kits were used to detect the levels of interleukin-6 (IL-6), tumor necrosis factor alfa (TNF-α), C-reactive protein (CRP), adrenocorticotropic hormone (ACTH), corticotropin-releasing hormone (CRH), and corticosterone (CORT) in serum, following the instructions provided by the manufacturer. Chronic PSD for 7 days resulted in higher levels of serum pro-inflammatory cytokines including IL-6, TNF-α, and CRP with P values < 0.001, 0.01 and 0.001, respectively
PMID: 30953642 -
Sample type: Plasma
Sample species: Rat
Review: The levels of serum C-reactive protein (CRP) were determined with ELISA kits. The contents of plasma MDA, TNF-α, and CRP were significantly higher in the moderate and high- dose PM2.5 exposure groups than in the controls (P < .05).
PMID: 27592525
靶点详情
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最新研究进展:CRP是C反应蛋白的简称,是一种急性反应蛋白。最新研究表明,CRP不仅可以作为心血管疾病等疾病的生物标志物,还与COVID-19感染的严重程度相关。此外,研究还发现CRP参与多种疾病的发生发展,包括癌症、自身免疫疾病和神经退行性疾病等。
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功能:Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
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基因功能参考文献:
- coordinated upregulation of genes encoding CRP, interleukin-6 (IL-6), HNF1alpha, and HNF4alpha in the livers and white adipose tissue (WAT) of chronic renal failure rats, as compared to the pair-fed and control animals. PMID: 29330688
- CRP: varied between DJOS and SHAM groups maintained on HF/HF, CD/CD, and CD/HF. PMID: 29849871
- Fibrinopeptide A induces CRP expression in Vascular Smooth Muscle Cells via Reactive Oxygen Species-ERK1/2/p38-NF-kappaB signal pathway. PMID: 29768263
- Knockdown of ZFAS1 or miR-150 overexpression effectively relieved AMI-induced myocardial infarction in AMI-1 week rats. The ZFAS1/miR-150 axis was involved in the molecular mechanism of AMI induced cardiomyocytes apoptosis via regulating CRP PMID: 28295592
- Epigallocatechin-3-gallate inhibits Ang II-induced CRP generation by interfering with AT1-ROS-ERK1/2 signal pathway in hepatocytes, preventing inflammation. PMID: 27492718
- Salvianolic acid B exerted anti-steatotic and anti-inflammatory effects against alcoholic liver injury by inducing SIRT1-mediated inhibition of CRP and ChREBP expression. PMID: 27989594
- Rosiglitazone inhibits angiotensin II-induced C-reactive protein generation by interfering with AT1-ROS-ERK1/2/JNK signal pathway and up-regulating PPARgamma expression in hepatocytes PMID: 26741265
- These findings provide evidence that, in the presence of high CRP levels, rosuvastatin exhibits significant anti-inflammatory, anti-oxidative, and renoprotective effects. PMID: 25536316
- Data show that palmitic acid significantly stimulated mRNA and protein expression of C-reactive protein, inducible nitric oxide synthase and tumor necrosis factor-alpha in vascular smooth muscle cells in time- and concentration-dependent manners. PMID: 25242580
- No correlation was found between CRP levels and microvascular or macrovascular dysfunction in adjuvant-induced arthritis. PMID: 25708190
- Homocysteine initiates an inflammatory response in VSMCs by stimulating CRP production via the NMDAr-ROS-ERK1/2/p38-NF-kappaB signal pathway. PMID: 25016361
- CRP and IgM depositions demonstrate a parallel time course throughout the reperfusion to hepatocellular damage, inflammatory response and activated complement deposition in an ischemia-reperfusion model. PMID: 24642533
- These demonstrate that aldosterone is able to induce CRP expression via MR-ROS-ERK1/2-NF-kappaB signal pathway in VSMCs, which provides a new evidence for the proinflammatory and proatherosclerotic effects of aldosterone. PMID: 25109280
- Monomeric CRP localized with inflammatory cells in infarcted myocardium. Intravenous pCRP administration significantly enhanced leukocyte rolling, adhesion, and transmigration via localized dissociation to mCRP in inflamed cremaster muscle. PMID: 24982116
- In older animals, there is depressed PAP expression related to a blunted inflammatory response in acute pancreatitis which is associated with worsened bacterial infiltration and higher C-reactive protein level PMID: 22807607
- PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats. PMID: 22201020
- C-reactive protein down-regulates endothelial nitric oxide synthase expression and promotes apoptosis in endothelial progenitor cells through receptor for advanced glycation end-products PMID: 22301267
- After attachment of polymeric CRP to the surface of necrotic cardiomyocytes, generation of monomeric CRP was demonstrated over a period of 18 h. PMID: 21856781
- This finding could indicate a possible role for CRP in the progression of small vessel disease in the brain and provide a therapeutic target PMID: 21840509
- C-reactive protein and tumour necrosis factor-alpha are elevated in collagen-induced arthritis in Dark Agouti rats PMID: 21633874
- Increased levels of CRP are an early sign of alcoholic fatty liver in rats. PMID: 21806828
- C-reactive protein exerts a proinflammatory action via Toll-like receptor 4-dependent signaling pathway (AT(1)R-p38 MAPK-TLR4-PKCalpha) in rat vascular smooth muscle cells. PMID: 20332628
- CRP may cause insulin resistance by increasing IRS-1 phosphorylation at Ser(307) and Ser(612) via JNK and ERK1/2, respectively, leading to impaired insulin-stimulated glucose uptake, GLUT4 translocation, and glycogen synthesis PMID: 17279354
- the Ala12 allele of PPARG2 may be an independent factor associated with low serum hs-CRP levels in Japanese females PMID: 17574226
- levels of C-reactive protein and adiponectin are improved after exercise training in diabetic rats PMID: 17660720
- CRP and IgM depositions correlated well with local complement activation, which suggests a role of these molecules in complement activation PMID: 17981193
- CRP levels and cardiovascular disease risk and the long term clinical outcome after acute coronary syndrome but such correlation has yet to be proven regarding CRP and atherosclerosis. PMID: 18254451
- C-reactive protein stimulates superoxide anion release and tissue factor activity in vivo PMID: 18621373
- CRP at clinically relevant concentrations (10 to 20 microg/mL) causes a disruption of the blood-brain barrier PMID: 19246692
- The results suggest that CRP contributes to memory loss and early phase of pathogenesis of Alzheimer disease PMID: 19263040
- The results demonstrate that ET-1 induces CPR production in VSMCs via ET(A) receptor followed by ROS and MAPK signal pathway. PMID: 19748488
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亚细胞定位:Secreted.
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蛋白家族:Pentraxin family
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组织特异性:Found in plasma.
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数据库链接:
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