Rabbit Interleukin 10,IL-10 ELISA Kit

产品详情

文献引用 (9) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子白介素10(IL-10)酶联免疫试剂盒（CSB-E06897Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是一种重要的免疫调节细胞因子。它可抑制多种促炎细胞因子的产生，从而发挥抗炎作用。目前关于IL10的研究聚焦于其在自身免疫病、感染性疾病等疾病中的作用机制，有望为相关疾病的治疗提供新靶点和新思路。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品广泛应用于免疫学研究领域，特别适用于评估炎症性疾病模型、感染免疫应答或免疫调节药物干预实验中IL-10的动态变化，为探索免疫调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：

IL10
Uniprot No.：

Q9TSJ4
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-98
1:2	Average %	96
	Range %	91-105
1:4	Average %	97
	Range %	92-110
1:8	Average %	93
	Range %	86-100

回收率：

The recovery of rabbit IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	89	80-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
亚细胞定位：

Secreted.
蛋白家族：

IL-10 family
数据库链接：

STRING: 9986.ENSOCUP00000019534

UniGene: Ocu.1796