Pig Interleukin 12,IL-12/P70 ELISA Kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪白介素12(IL-12/P70)酶联免疫试剂盒（CSB-E13288p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL12/P70含量。IL12/P70是一种重要的靶点。它在免疫系统中发挥关键作用，能够调节免疫细胞功能，参与机体炎症反应和免疫应答过程。相关研究主要聚焦于其信号传导通路和与疾病的关联机制，有望为炎症性疾病、肿瘤等的治疗提供新策略。试剂盒检测范围为0.45 ng/mL-30 ng/mL，灵敏度为0.11 ng/mL。适用于猪类动物模型中的免疫调控机制研究、疫苗效力评估、感染性疾病或炎症反应模型中细胞因子网络的动态分析；适用于畜牧兽医研究、免疫毒理学实验及生物制剂开发等领域的定量分析需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
缩写：

IL-12/P70
Uniprot No.：

Q29053
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.45 ng/mL-30 ng/mL
灵敏度：

0.11 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	84
	Range %	80-89
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

KEGG: ssc:397053

STRING: 9823.ENSSSCP00000012501

UniGene: Ssc.13