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中文名称:小鼠促肾上腺皮质激素(ACTH)酶联免疫试剂盒
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货号:CSB-E06874m
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:
The mouse ACTH ELISA kit (CSB-E06874m) is designed for the quantitative measurement of mouse ACTH protein in serum and plasma. It quantitates mouse ACTH with 4.68 pg/ml sensitivity and shows excellent specificity for mouse ACTH. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated ACTH antibody that recognizes the analyte bound by the immobilized ACTH antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of ACTH in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse ACTH in the sample.
ACTH is a tropic hormone synthesized in the basophilic cells of the anterior pituitary gland of the brain. It mainly functions to stimulate the production and release of cortisol from the cortex of the adrenal gland. ACTH is associated with Addison's disease, Cushing's syndrome, and Cushing's disease. It plays a role in glucose metabolism and immune function. Additionally, ACTH also participates in bone resorption, catabolism of proteins, anabolic effects on muscle and bones, as well as stimulation of spermatogenesis in men.
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缩写:ACTH
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种属:Mus musculus (Mouse)
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样本类型:serum, plasma
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检测范围:18.75 pg/mL-1200 pg/mL
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灵敏度:4.68 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 80-105 1:2 Average % 96 Range % 85-104 1:4 Average % 98 Range % 92-104 1:8 Average % 96 Range % 82-110 -
回收率:
The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 84-102 EDTA plasma (n=4) 98 90-105 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1200 3.134 3.126 3.130 2.988 600 2.537 2.532 2.535 2.393 300 1.754 1.765 1.760 1.618 150 1.053 1.050 1.052 0.910 75 0.651 0.647 0.649 0.507 37.5 0.443 0.435 0.439 0.297 18.75 0.273 0.265 0.269 0.127 0 0.140 0.144 0.142 -
数据处理:
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货期:3-5 working days
引用文献
- Excessive free fatty acid sensing in pituitary lactotrophs elicits steatotic liver disease by decreasing prolactin levels X Ji,Cell reports,2024
- Single-cell transcriptomic analysis reveals rich pituitary–Immune interactions under systemic inflammation T Yan,PLoS biology,2023
- Dendrobium officinale polysaccharides alleviate depression-like symptoms via regulating gut microbiota-neuroinflammation in perimenopausal mice QP Zhang,Journal of Functional Foods,2021
- Intranasal Oxytocin Administration Ameliorates DSS-induced Abnormal Stress-related Behavior and Intestinal Inflammation in an IBD Mouse Model C Liu,Authorea Preprints,2020
- Magnolol abrogates chronic mild stress-induced depressive-like behaviors by inhibiting neuroinflammation and oxidative stress in the prefrontal cortex of mice Cheng J.et al,Int Immunopharmacol.,2018
- Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017
产品评价
样品类型:血清
样品信息:小鼠
稀释比:其他 1:4
产品评价: 使用CSB-E06874m试剂盒检测小鼠血清ACTH水平,稀释4倍后正常与模型组及各给药组大部分均在标曲范围内,但如果样品充足可考虑1:1稀释或使用原液,换算稀释倍数后原液仍在标曲范围内。全部实验在6小时内完成,检测结果较稳定,标曲良好.
By 兰老师
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