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Mouse adrenocorticotropic hormone (ACTH) ELISA kit

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  • 产品描述:

    The mouse ACTH ELISA kit (CSB-E06874m) is designed for the quantitative measurement of mouse ACTH protein in serum and plasma. It quantitates mouse ACTH with 4.68 pg/ml sensitivity and shows excellent specificity for mouse ACTH. It uses the bi-antibody sandwich enzyme immunoassay technique. This assay employs a biotin-conjugated ACTH antibody that recognizes the analyte bound by the immobilized ACTH antibody, forming an antibody-analyte-antibody complex. The immune complex is further detected by avidin-conjugated HRP. The TMB solution is added into the wells and turns blue and finally turns yellow after the addition of the stop solution. Solution color develops in proportion to the amount of ACTH in the sample. The O.D. value is measured using a microplate reader at 450 nm and is used to determine the concentration of the mouse ACTH in the sample.

    ACTH is a tropic hormone synthesized in the basophilic cells of the anterior pituitary gland of the brain. It mainly functions to stimulate the production and release of cortisol from the cortex of the adrenal gland. ACTH is associated with Addison's disease, Cushing's syndrome, and Cushing's disease. It plays a role in glucose metabolism and immune function. Additionally, ACTH also participates in bone resorption, catabolism of proteins, anabolic effects on muscle and bones, as well as stimulation of spermatogenesis in men.

  • 缩写:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    18.75 pg/mL-1200 pg/mL
  • 灵敏度:
    4.68 pg/mL
  • 反应时间:
  • 样本体积:
  • 检测波长:
    450 nm
  • 研究领域:
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  • 测定方法:
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    1:1Average %92
    Range %80-105
    1:2Average %96
    Range %85-104
    1:4Average %98
    Range %92-104
    1:8Average %96
    Range %82-110
  • 回收率:
    The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9584-102
    EDTA plasma (n=4)9890-105
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    12003.134 3.126 3.130 2.988
    6002.537 2.532 2.535 2.393
    3001.754 1.765 1.760 1.618
    1501.053 1.050 1.052 0.910
    750.651 0.647 0.649 0.507
    37.50.443 0.435 0.439 0.297
    18.750.273 0.265 0.269 0.127
    00.140 0.144 0.142  
  • 数据处理:
  • 货期:
    3-5 working days