Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

产品详情

文献引用 (3) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Mouse CK 18-M30 ELISA Kit was designed for the quantitative measurement of Mouse CK 18-M30 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.
缩写：

CK 18-M30
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 μIU/mL-1000 μIU/mL
灵敏度：

3.9 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
Range %	89-104
1:200	Average %	95
Range %	90-100
1:400	Average %	92
Range %	87-101
1:800	Average %	93
Range %	90-97

回收率：

The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	90-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

■ 客户评论

Tested application: ELISA
Test sample: Plasma (anticoagulant)
Species: Mouse
Review: Repeatability is very good, so order more kits for more tests.
Tested application: ELISA
Test sample: Serum
Species: Mouse
Review:Test results were like we expect. Duplicates pretty close.
Sample type: Serum, Plasma
Sample species: Mouse
Review: We have used this kit for the same model before, it worked as expected. But this lot does not working as consistent as previously.