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Mouse Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

  • 中文名称:
    小鼠细胞角蛋白18-M30(CK 18-M30)酶联免疫试剂盒
  • 货号:
    CSB-E14265m
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Mouse CK 18-M30 ELISA Kit was designed for the quantitative measurement of Mouse CK 18-M30 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μIU/mL-1000 μIU/mL and the sensitivity is 3.9 μIU/mL.

  • 缩写:
    CK 18-M30
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 μIU/mL-1000 μIU/mL
  • 灵敏度:
    3.9 μIU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 96  
    Range % 89-104  
    1:200 Average % 95  
    Range % 90-100  
    1:400 Average % 92  
    Range % 87-101  
    1:800 Average % 93  
    Range % 90-97  
  • 回收率:
    The recovery of mouse CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 96 90-104  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µIU/ml OD1 OD2 Average Corrected  
    1000 2.317 2.217 2.267 2.164  
    500 1.467 1.367 1.417 1.314  
    250 0.797 0.777 0.787 0.684  
    125 0.412 0.392 0.402 0.299  
    62.5 0.268 0.258 0.263 0.160  
    31.2 0.199 0.189 0.194 0.091  
    15.6 0.165 0.164 0.165 0.062  
    0 0.103 0.102 0.103    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

问答及客户评论

 客户评论
  • Tested application: ELISA
    Test sample: Plasma (anticoagulant)
    Species: Mouse
    Review: Repeatability is very good, so order more kits for more tests.
  • Tested application: ELISA
    Test sample: Serum
    Species: Mouse
    Review:Test results were like we expect. Duplicates pretty close.
  • Sample type: Serum, Plasma
    Sample species: Mouse
    Review: We have used this kit for the same model before, it worked as expected. But this lot does not working as consistent as previously.