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Mouse Fibrinogen Degradation Product,FDP ELISA Kit

  • 中文名称:
    小鼠血纤蛋白原降解产物(FDP)酶联免疫试剂盒
  • 货号:
    CSB-E07941m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

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  • 产品描述:
    该小鼠血纤蛋白原降解产物(FDP)酶联免疫试剂盒(货号:CSB-E07941m)基于竞争法原理,可定量检测血清或血浆样本中1.56 ng/mL至100 ng/mL浓度范围的FDP水平。FDP作为纤溶系统激活的重要标志物,其水平变化与凝血功能异常、血栓性疾病进展及组织损伤修复等病理生理过程密切相关,常用于心血管疾病、肿瘤转移或炎症反应等机制研究。CUSABIO试剂盒采用预包被特异性抗体的微孔板,通过样本中的FDP与标记物竞争性结合,结合酶标二抗显色系统实现精准定量,适用于评估小鼠模型中溶栓治疗效果、血栓形成动态监测或纤维蛋白代谢相关研究,为纤溶系统调控及抗凝药物开发提供可靠的实验工具。试剂盒操作流程标准化,配备完整质控组分,满足体外科研实验对重复性和灵敏度的要求。
  • 别名:
    N/A
  • 缩写:
    FDP
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma
  • 检测范围:
    1.56 ng/mL-100 ng/mL
  • 灵敏度:
    0.78 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 85-97  
    1:200 Average % 93  
    Range % 88-98  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 88  
    Range % 84-93  
  • 回收率:
    The recovery of mouse FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 92 88--95  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.108 0.101 0.105    
    50 0.213 0.226 0.220    
    25 0.427 0.411 0.419    
    12.5 0.785 0.753 0.769    
    6.25 1.224 1.147 1.186    
    3.12 1.679 1.729 1.704    
    1.56 1.894 1.929 1.912    
    0 2.305 2.412 2.359    
  • 数据处理:
  • 货期:
    3-5 working days

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