Human lipolysaccharide binding protein,LBP ELISA Kit
CUSABIO’s Human lipopolysaccharide-binding protein (LBP) ELISA Kit is suitable for the quantitative detection of LBP content in human serum, plasma, or tissue homogenates. LBP is a carrier protein of the endotoxins' recognition by the host. It is produced by hepatocytes and released into the bloodstream as a consequence of Gram-negative infection. LBP conveys LPS to the cellular surface, forming a ternary complex with a cluster of CD14. CD14 facilitates the transfer of LPS to the TLR4/MD2 complex, activating LPS-TLR4 signaling that elicits proinflammatory responses. It is significantly up-regulated during acute-phase reaction following the initial inflammatory responses, promoting, along with (bactericidal/permeability-increasing protein), non-inflammatory clearance of LPS, and ultimate resolution of LPS-induced inflammation. Additionally, LBP mediates endothelial dysfunction and is required for apoptosis in tubular cells.
The detection mechanism of this kit is based on the sandwich ELISA technique and enzyme-substrate chromogenic reaction. The formed pre-coated LBP antibody/LBP/Biotin-conjugated LBP antibody complex is labeled by HRP-avidin and then develops a color reaction after the addition of The TMB substrate solution. The color intensity can be measured at 450 nm via a microplate reader.
BPI fold containing family D, member 2 ELISA Kit; Bpifd2 ELISA Kit; LBP ELISA Kit; LBP_HUMAN ELISA Kit; LBP1 ELISA Kit; Lipopolysaccharide binding protein ELISA Kit; Lipopolysaccharide-binding protein ELISA Kit; LPS binding protein ELISA Kit; Ly88 ELISA Kit; MGC22233 ELISA Kit; OTTHUMP00000030965 ELISA Kit; RP23-407H16.4 ELISA Kit
Homo sapiens (Human)
serum, plasma, tissue homogenates
0.625 μg/mL-40 μg/mL
|Intra-assay Precision (Precision within an assay): CV%<8%|| || || |
|Three samples of known concentration were tested twenty times on one plate to assess. || |
|Inter-assay Precision (Precision between assays): CV%<10%|| || || |
|Three samples of known concentration were tested in twenty assays to assess. || || |
| || || || || || || |
|To assess the linearity of the assay, samples were spiked with high concentrations of human LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
| ||Sample||Serum(n=4)|| |
|1:1||Average %||97|| |
|Range %||92-102|| |
|1:2||Average %||95|| |
|Range %||91-99|| |
|1:4||Average %||99|| |
|Range %||96-108|| |
|1:8||Average %||94|| |
|Range %||89-98|| |
|The recovery of human LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|| |
|Serum (n=5) ||99||92-108|| |
|EDTA plasma (n=4)||84||80-88|| |
| || || || || || || |
| || || || || || || |
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|40||2.795 ||2.694 ||2.745 ||2.613 || |
|20||2.232 ||2.131 ||2.182 ||2.050 || |
|10||1.464 ||1.443 ||1.454 ||1.322 || |
|5||0.812 ||0.823 ||0.818 ||0.686 || |
|2.5||0.425 ||0.415 ||0.420 ||0.288 || |
|1.25||0.300 ||0.311 ||0.306 ||0.174 || |
|0.625||0.222 ||0.218 ||0.220 ||0.088 || |
|0||0.132 ||0.131 ||0.132 || || |
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human LBP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled LBP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Test tubes for dilution
3-5 working days
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Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
- This study shows a remarkable reversal of amyloid fibrin formation by LBP addition to the plasma of Parkinson's Disease patients . PMID: 29494603
- Serum LBP levels are associated with arterial stiffness, independent of obesity and traditional cardiovascular risk factors, especially in men with type 2 diabetes. PMID: 28486964
- novel observation that sCD14 compared with lipopolysaccharide binding protein, offers a preferred target to ameliorate TLR especially TLR4-induced inflammation and insulin resistance in human obesity and metabolic syndrome PMID: 26880233
- LBP, an endotoxemia associated protein might be used as an inflammatory biomarker of both infectious and non-infectious origins in HCV-infected subjects PMID: 28107471
- Data show that after matching for gender, age, and body mass index (BMI), serum lipopolysaccharide-binding protein (LBP) does not improve prediction of the development of type 2 diabetes mellitus (T2DM) independently. PMID: 25753130
- The main findings of this study are that, in acute stroke patients, levels of LBP, IL-10, IL-6 and CRP show a different time course in patients with and without post-stroke infection. PMID: 25613713
- Serum LBP level is significantly elevated in polycystic ovary syndrome women and is associated with insulin resistance. PMID: 26799617
- LBP serves not only as an extracellular LPS shuttle but in addition facilitates intracellular transport of LPS. PMID: 26804480
- LBP level was not significantly different in neutropenic systemic inflammatory response syndrome patients and sepsis patients. PMID: 26046926
- Report increased secretion of Fetuin A, LBP and HMGB-1 from subcutaneous adipose tissue in metabolic syndrome. PMID: 25978344
- Low levels of microbial translocation marker LBP are associated with sustained viral response after anti-HCV treatment in HIV-1/HCV co-infected patients PMID: 25785448
- serum level elevated in late-onset neonatal sepsis in very low birth weight infants PMID: 25014514
- Adjusting for body mass index and waist circumference, LBP levels remained significantly increased in metabolic syndrome and increased with increasing numbers of MetS risk factors. PMID: 25063948
- study suggested that elevated plasma LBP was associated with an increased risk of developing MetS among middle-aged and older Chinese, especially in normal-weight individuals. PMID: 24906952
- In preterm neonates born to asymptomatic women with PPROM, LBP in cord blood at delivery is an excellent diagnostic biomarker of Fetal inflammatory response syndrome/funisitis with prognostic potential. PMID: 24335151
- report pre-ART IL-6 and LBP levels as well as IL-6, LBP and I-FABP levels during IRIS-event as potential biomarkers in TB-IRIS PMID: 24312369
- In childnre, LBP was independently associated with BMI and with measures of obstructive sleep apnea severity as well as with metabolic dysfunction, particularly insulin resistance as indicated by the homeostasis model assessment of insulin resistance. PMID: 24276451
- Suggest that the serum LBP concentration constitutes a reliable biologic marker for the diagnosis of a febrile urinary tract infection in children. PMID: 23463341
- Mutations in the lipopolysaccharide binding protein impair innate immunity. PMID: 24120359
- Serum LBP concentrations increased with age. Overweight, obesity, and having metabolic syndrome (particularly, low HDL cholesterol levels) were associated with higher LBP concentrations PMID: 23349936
- expression in oral keratinocytes is stimulated by Porphyromonas gingivalis LPS PMID: 22736337
- Human lipopolysaccharide-binding protein (LBP) and CD14 independently deliver triacylated lipoproteins to Toll-like receptor 1 (TLR1) and TLR2 and enhance formation of the ternary signaling complex PMID: 23430250
- LBP is a surrogate marker of microbial translocation in association with physical functioning of older adults. PMID: 22960476
- Association of lipopolysaccharide-binding protein gene polymorphisms with cerebral infarction in a Chinese population PMID: 22476641
- our study demonstrated that an elevated LBP level of >15000 ng/ml may serve as a biomarker for the prediction and monitoring of aGVHD. PMID: 22552880
- During the first 14 days of postoperative sepsis, LBP plasma concentrations showed a time course that was very similar to CRP with a high concordance in the pattern of day-to-day changes PMID: 21901123
- The availability of commercial methods for the automated measurement of the soluble CD14 subtype presepsin and lipopolysaccharide binding protein represent a challenge for the evaluation in clinical practice of reliable markers of neonatal sepsis. PMID: 21740312
- Aseptic trauma primes the innate immune system for the posttraumatic release of lipopolysaccharide binding protein and sCD14 PMID: 21722015
- Cytokine concentrations in amniotic fluid during the mid-trimester did not differ with parity or fetal gender. IL-6, IL-8, and LBP levels appeared stable with gestational week (GW), whereas GW significantly influenced TNF-alpha concentrations. PMID: 21702700
- LBP measurements performed shortly after preterm premature rupture of membranes, are not of value either in the prediction of newborn's infection, or in the prognosis of latency period duration. PMID: 21353369
- This study highlights the LBP rs2232596 and CD14 rs4914 polymorphisms as biomarkers for elevated colorectal carcinoma susceptibility in the Chinese Han population PMID: 21633598
- High LBP is associated with Crohn's disease. PMID: 20865702
- Elevated circulating LBP was associated with obesity, metabolic syndrome, and type 2 diabetes in apparently healthy Chinese. PMID: 20530747
- The association of the polymorphisms c.291C>T and c.613A>G suggest a role of LBP in the disease manifestation of infective endocarditis. PMID: 19560454
- Serial LBP serum measurements may offer a clinically useful biomarker for identification of patients with severe sepsis having the worst outcomes and the highest probability of developing sepsis-induced ARDS PMID: 19718443
- carboxyl-terminal domain of these closely related endotoxin-binding proteins dictates the route and host responses to complexes they form with endotoxin. PMID: 11773072
- a new and reliable infection marker after kidney transplantation PMID: 11976738
- Identification of single amino acid residues essential for the binding of lipopolysaccharide (LPS) to LPS binding protein (LBP) residues 86-99 by using an Ala-scanning library PMID: 11991204
- plasma factor LBP and cell surface receptor CD14 were necessary for LPS activation of p38, which was tightly associated with LPS priming of the PMN respiratory burst PMID: 12117913
- Besides a role in the detoxification of bacterial toxin present in the circulation, LBP-chylomicron complexes may be part of a local defense mechanism of the intestine against translocated bacterial toxin. PMID: 12538700
- innate immune recognition of LTA via LBP, CD14, and TLR-2 represents an important mechanism in the pathogenesis of systemic complications in the course of infectious diseases brought about by Gram-positive pathogens. while TLR-4 and MD-2 are not involved. PMID: 12594207
- Single nucleotide polymorphism of the LBP gene is not assciated with complicated sepsis after trauma. PMID: 12615620
- data support the hypothesis that lipopolysaccharide binding protein can inhibit cell responses to lipopolysaccharide(LPS) by inhibiting LPS transfer from membrane CD14 to the Toll-like receptor 4-MD-2 signaling receptor PMID: 12754215
- plays an essential role in the innate immune response to Gram-positive pneumococci PMID: 12932360
- In critically ill neonates aged over 48 h and children lipopolysaccharide binding protein is a better marker than procalcitonin, interleukin-6 and C-reactive protein. PMID: 15127192
- Dual role of LBP and CD14 in initiation of proinflammatory signaling and clearance or neutralization of LPS. PMID: 15618154
- studies suggest that SP-A could contribute to modulate Re-LPS responses by altering the competence of the LBP-CD14 receptor complex PMID: 15932345
- LBP mediates the fusion of lipid membranes and LPS aggregates. PMID: 16303759
- Human pulmonary LBP acts as an important modulator of the LPS response in the respiratory tract in vivo. PMID: 16493079
- Significantly elevated serum concentrations of LBP and sCD14 are found in severe sepsis patients. PMID: 16512634
Secreted. Cytoplasmic granule membrane.
BPI/LBP/Plunc superfamily, BPI/LBP family
Detected in blood serum (at protein level).
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