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Human SARS-CoV-2 N IgG Antibody ELISA Kit

  • 中文名称:
    人新型冠状病毒核蛋白(SARS-CoV-2 N) IgG抗体 ELISA Kit
  • 货号:
    CSB-EL3325HU
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    CUSABIO's Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

    Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.

  • 别名:
    N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
  • 缩写:
    SARS-CoV-2 N Ab (IgG)
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Infectious Diseases
  • 测定原理:
    qualitative
  • 测定方法:
    Indirect
  • 说明书:
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%  
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%  
    Three samples of known concentration were tested in twenty assays to assess.
  • 标准曲线:
    Test parameter specification test result
    Positive control ≥0.6 0.867
    Negative control ≤0.25 0.184
    Positive rate 20,Positive 100%
    Negative rate 20,Negative 100%
  • 本试剂盒所含材料:
      • A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
      • Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
      • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
      • HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
      • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
      • Sample Diluent (2 x 20 ml) --Dilute the sample solution.
      • Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
      • TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
      • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
      • An Instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 50-300ul multi-channel micropipette
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Pipette tips
      • Timer
      • Test tubes for dilution.

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  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
  • 亚细胞定位:
    Virion. Host cytoplasm.
  • 蛋白家族:
    Betacoronavirus nucleocapsid protein family