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Human Keratin, type I cytoskeletal 18 fragment (K18-Asp396)ELISA Kit

  • 中文名称:
    人细胞角蛋白18-M30(CK 18-M30)酶联免疫试剂盒
  • 货号:
    CSB-E13696h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human CK 18M30 ELISA Kit was designed for the quantitative measurement of Human CK 18M30 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 mIU/mL-1000 mIU/mL and the sensitivity is 3.9 mIU/mL .

  • 别名:
    N/A
  • 缩写:
    CK 18M30
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    15.6 mIU/mL-1000 mIU/mL
  • 灵敏度:
    3.9 mIU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-94  
    1:2 Average % 86  
    Range % 81-92  
    1:4 Average % 94  
    Range % 90-99  
    1:8 Average % 89  
    Range % 83-96  
  • 回收率:
    The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-100  
    EDTA plasma (n=4) 99 94-105  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    1000 2.577 2.552 2.565 2.443  
    500 2.056 2.016 2.036 1.914  
    250 1.267 1.202 1.235 1.113  
    125 0.683 0.648 0.666 0.544  
    62.5 0.379 0.405 0.392 0.270  
    31.2 0.294 0.279 0.287 0.165  
    15.6 0.201 0.199 0.200 0.078  
    0 0.117 0.126 0.122    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

问答及客户评论

 常见问题解答
Q:

请问人细胞角蛋白18片段Asp396(K18-Asp396)ELISA Kit(CSB-E13696h)是评估细胞凋亡的试剂盒吗?评估总细胞死亡(凋亡+坏死)的补充指标是什么?

A:
检测M30可代表肝细胞凋亡水平。当肝细胞发生坏死和凋亡时,其细胞膜完整性被破坏,CK18大量释放到外周血中。可溶性CK18总量在凋亡细胞和坏死细胞中均有表达,且通过M65均可检测到。即研究可与CK18-M30和CK18-M65结合进行。 为此,我们有相应的试剂盒,即CSB-E13696h、 CSB-E09172h(试剂盒是为全长CK-18设计的)。这两种试剂盒已被一些文章引用。