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Human Carboxymethyl lysine,CML ELISA Kit

  • 中文名称:
    人羧甲基赖氨酸(CML)酶联免疫试剂盒
  • 货号:
    CSB-E12798h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Human CML ELISA Kit was designed for the quantitative measurement of Human CML protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL .

  • 缩写:
    CML
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    62.5 pg/mL-4000 pg/mL
  • 灵敏度:
    15.6 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %82-95
    1:2Average %94
    Range %90-100
    1:4Average %95
    Range %91-105
    1:8Average %94
    Range %85-100
  • 回收率:
    The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-98
    EDTA plasma (n=4)9491-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.372 2.252 2.312 2.162
    20001.775 1.660 1.718 1.568
    10001.160 1.142 1.151 1.001
    5000.712 0.699 0.706 0.556
    2500.493 0.456 0.475 0.325
    1250.342 0.329 0.336 0.186
    62.50.264 0.249 0.257 0.107
    00.153 0.147 0.150  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

问答及客户评论

 常见问题解答
Q:

Does this kit measure the total or protein bound cml?

A:
Thanks for your inquiry. CSB-E12798h detects protein bound CML.Pls let me know if you have any further questions. Thank you.
Q:

We have planned to assess the abundance of cml in protein samples, collected from cell cultures, using the human CML ELISA kit CSB-E12798h. What kind of lysis buffer is recommended to use to collect CML in cell samples? We are used to lyse cell with T-PER/Protease inhibitor cocktail or Igepal at 0.5%. Are they compatible with the purchased CML Elisa kit (CSB-E12798h)

A:
Thanks for your inquiry.
From your description, you want to make detection on cell lysis, here advice you to use the sample method on our attached manual.
As for the method you mentioned, so sorry that it is not verified from our side.
If your sample has been prepared, pls arrange preliminary experiment to check.
The enzyme proportion should be combined with the enzyme preparation protocol.
And dear, we have 24T trial kit, if you have interest, advice you to have this small size for try.
Any question, pls feel free to contact me.Pls let me know if you have any further questions. Thank you.