Guinea pig interleukin 5,IL-5 ELISA Kit

产品详情

文献引用 (2) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

豚鼠白介素5(IL-5)酶联免疫试剂盒（CSB-E12064Gp）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL5含量。IL5是重要的治疗靶点。它主要由活化的T淋巴细胞产生，能促进嗜酸性粒细胞的生长、分化和存活。针对IL5的研究主要围绕阻断其与受体结合，以减少嗜酸性粒细胞数量，进而治疗哮喘、嗜酸性粒细胞增多症等嗜酸性粒细胞相关疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于豚鼠疾病模型的免疫机制研究，例如哮喘、寄生虫感染等实验中的炎症因子动态监测，或用于评估免疫调节药物对IL - 5通路的影响。组织匀浆样本可用于分析肺、骨髓等靶器官的局部IL - 5表达水平，为科研人员提供稳定可靠的定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL5Interleukin-5 ELISA Kit; IL-5 ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
缩写：

IL5
Uniprot No.：

O08987
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	94
	Range %	89-99
1:4	Average %	103
	Range %	98-108
1:8	Average %	100
	Range %	91-105

回收率：

The recovery of guinea pig IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-97
EDTA plasma (n=4)	92	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
亚细胞定位：

Secreted.
蛋白家族：

IL-5 family
数据库链接：

STRING: 10141.ENSCPOP00000004355