Dog interleukin 12 (IL-12/P70) ELISA kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬白介素12(IL-12/P70)酶联免疫试剂盒（CSB-EQ027628DO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL12/P70含量。IL12/P70是一种重要的靶点。它在免疫调节中起关键作用，能促进Th1细胞分化、增强NK细胞活性等。研究主要围绕其参与的免疫信号通路，探索通过调控它来治疗如自身免疫性疾病、肿瘤等，有望为相关疾病治疗带来新策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于犬类免疫机制研究，包括炎症性疾病模型建立、疫苗免疫效果评估或感染后细胞因子动态监测等科研领域，可为动物免疫学、传染病学及比较医学研究提供可靠的实验数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL12/P70, Interleukin-12/P70
缩写：

IL-12/P70
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	87
	Range %	84-93
1:4	Average %	97
	Range %	91-106
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	97	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.