活性蛋白 > 其他 > Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active)
产品名称:
Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active) IN STOCK
货期:
3-7 工作日
uniprot号:
Q9NZQ7
货号:
CSB-MP878942HU1
规格:
￥1800
图像:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
内毒素:
Less than 1.0 EU/ug as determined by LAL method.
生物活性:
Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
参考文献:
"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
Dong H., Zhu G., Tamada K., Chen L.
Nat. Med. 5:1365-1369(1999)
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
蛋白储存:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
表达区域:
19-238aa
使用注意:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
