活性蛋白 > 其他 > Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active)
  • 产品名称:

    Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active) IN STOCK

  • 货期:
    3-7 工作日
  • uniprot号:
    Q9NZQ7
  • 货号:
    CSB-MP878942HU1
  • 规格:
    ¥1800
  • 图像:


    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.

  • 测活实验手册:
  • 分子结构:
  • 基因名称:
    CD274
  • 别名:
    PD-L1 (PDCD1 ligand 1) (Programmed death ligand 1) (hPD-L1) (B7 homolog 1) (B7-H1) (CD274)
  • 种属:
    Homo sapiens (Human)
  • 蛋白描述:
    Partial
  • 分子量:
    52.7 kDa
  • 标签信息:
    C-terminal FC-tagged
  • 来源:
    Mammalian cell
  • 纯度:
    Greater than 95% as determined by SDS-PAGE.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 内毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
  • 参考文献:
    "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
    Dong H., Zhu G., Tamada K., Chen L.
    Nat. Med. 5:1365-1369(1999)
  • 氨基酸序列:
    FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLL
    KDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAE
    GYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPE
    LPLAHPPNER
  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • 简介:
    Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
    The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
  • 蛋白储存:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
  • 表达区域:
    19-238aa
  • 使用注意:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 研究领域:
    Cancer