活性蛋白 > 其他 > Recombinant Human Novel Coronavirus Nucleoprotein(N)
  • 产品名称:

    Recombinant Human Novel Coronavirus Nucleoprotein(N) IN STOCK

  • 货期:
    3-7 工作日
  • uniprot号:
    P0DTC9
  • 货号:
    CSB-EP3325GMY
  • 规格:
  • 图像:


    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.



    Western Blot

    Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)

    SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)

    Secondary

    Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific(CSB-PA160506) at 1/20000 dilution

    Predicted band size: 48 kDa

    Observed band size: 55 kDa



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml.



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml.


  • 测活实验手册:
  • 基因名称:
    N (Nucleoprotein)
  • 别名:
    Nucleocapsid protein, NC, protein N, N
  • 种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 蛋白描述:
    Full Length
  • 分子量:
    48.0kDa
  • 标签信息:
    N-terminal 6xHis-tagged
  • 来源:
    E.coli
  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml.
  • 氨基酸序列:
    MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQ
    GVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALN
    TPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARM
    AGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPE
    QTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQV
    ILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
  • 说明书:

    注:产品说明书可能有改进,请直接与CUSABIO产品专员联系,索取最新版说明书!

  • 蛋白储存:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
  • 表达区域:
    1-419aa
  • 使用注意:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 状态:
    Lyophilized powder
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 研究领域:
    Microbiology