抗体 > 多克隆抗体 > SOX2 Antibody
  • 产品类型:
    Polyclonal Antibody
  • 产品名称:

    SOX2 Antibody

  • 货号:
    CSB-PA16539A0Rb
  • 规格:
    ¥1200
  • 别名:
    ANOP3 antibody; cb236 antibody; Delta EF2a antibody; lcc antibody; MCOPS3 antibody; MGC148683 antibody; MGC2413 antibody; RGD1565646 antibody; Sex determining region Y box 2 antibody; SOX 2 antibody; Sox2 antibody; SOX2_HUMAN antibody; SRY (sex determining region Y) box 2 antibody; SRY box containing gene 2 antibody; SRY related HMG box 2 antibody; SRY related HMG box gene 2 antibody; SRY-box 2 antibody; Transcription factor SOX 2 antibody; Transcription factor SOX-2 antibody; ysb antibody
  • 种属:
    Homo sapiens (Human)
  • 免疫原:
    Recombinant Human Transcription factor SOX-2 protein (1-317AA)
  • 宿主:
    Rabbit
  • 种属反应性:
    Human, Mouse
  • 应用范围:
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • 背景:
    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal By similarity. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation
  • 克隆类型:
    Polyclonal
  • 抗体亚型:
    IgG
  • 纯化方式:
    >95%, Protein G purified
  • 标记方式:
    Non-conjugated
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式:
    Liquid
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 图片:

    Western blot

    All lanes: Transcription factor SOX-2 antibody at 2μg/ml + Mouse kidney tissue

    Secondary

    Goat polyclonal to rabbit IgG at 1/15000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

    Western Blot

    All lanes: Transcription factor SOX-2 antibody at 6μg/ml + 293T whole cell lysate

    Secondary

    Goat polyclonal to rabbit IgG at 1/15000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

    IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    Western Blot

    Positive WB detected in: Mouse brain tissue

    All lanes: SOX2 antibody at 3.5μg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 34 kDa

    Observed band size: 34 kDa

    Western Blot

    Positive WB detected in: Zebrafish 40μg, 20μg, 10μg

    All lanes: Sox2 antibody at 3μg/ml

    Secondary

    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

    IHC image of CSB-PA16539A0Rb diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    Immunofluorescence staining of HepG2 cells with CSB-PA16539A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • 说明书:
  • Accession No.:
    P48431
  • 指标名称:
    SOX2
  • 研究领域:
    Neuroscience
  • 引用文献:
    1. Quadrella incana (Capparaceae) Leaf Extract Enhances Proliferation and Maintenance of Neural Stem/Progenitor Cells through Upregulating Glycolytic Flux and Redox Potential , M Kang,Oxid Med Cell Longev,2020
    2. Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/β-catenin pathways to promote human pluripotent stem cell self-renewal , Kim JY.et al,Sci Rep,2018