试剂盒 > 酶联免疫试剂盒 > 绵羊花生四烯酸(AA)ELISA kit
    • ELISA Kit
    • 绵羊花生四烯酸(AA)ELISA kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    绵羊花生四烯酸(AA)ELISA kit

  • 英文名称:

    Sheep arachidonic Acid(AA) ELISA kit

  • 货号:
    CSB-EQ027590SH
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Sheep
  • 其他种属:
  • 待测物名称:
    arachidonic Acid(AA)
  • 缩写:
    AA
  • 蛋白功能1:
    others
  • 检测范围:
    15.625 ng/ml-1000 ng/ml
  • 灵敏度:
    13.797 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    1000 0.122 0.120 0.121
    500 0.166 0.158 0.162
    250 0.232 0.241 0.237
    125 0.337 0.344 0.341
    62.5 0.528 0.510 0.519
    31.25 0.941 0.955 0.948
    15.625 1.471 1.522 1.497
    0 2.326 2.419 2.373
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:100 Average % 96
    Range % 89-100
    1:200 Average % 90
    Range % 85-95
    1:400 Average % 100
    Range % 94-104
    1:800 Average % 105
    Range % 97-110
  • 回收率:
    The recovery of sheep AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 95-106
    EDTA plasma (n=4) 102 96-107
  • 研究领域:
    Biochemicals