试剂盒 > 酶联免疫试剂盒 > 大鼠组织因子途径抑制物(TFPI)ELISA Kit
    • ELISA Kit
    • 大鼠组织因子途径抑制物(TFPI)ELISA Kit
    • This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed.[provided by RefSeq]
    • UniProtP19761
    • HGNC11760
    • RGD61914
    • MGI1095418
    • OMIM152310
    • CUSABIOCSB-E08261r
  • 产品类型:
    ELISA Kit
  • 产品名称:

    大鼠组织因子途径抑制物(TFPI)ELISA Kit

  • 英文名称:

    Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

  • 货号:
    CSB-E08261r
  • 别名:
    EPI, LACI, TFI, TFPI1, OTTHUMP00000163472|OTTHUMP00000205431|anti-convertin|extrinsic pathway inhibitor|lipoprotein-associated coagulation inhibitor|tissue factor pathway inhibitor
  • 规格:
    96T
  • 中文价格:
    3900
  • 种属:
    Rat
  • 其他种属:
  • 待测物名称:
    tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • 缩写:
    TFPI
  • 蛋白功能1:
    Blood Coagulation
  • 蛋白功能3:
    Blood coagulation
  • 检测范围:
    7.8 ng/ml-500 ng/ml
  • 灵敏度:
    1.95 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    500 2.596 2.474 2.535 2.424  
    250 1.978 1.902 1.940 1.829  
    125 1.172 1.096 1.134 1.023  
    62.5 0.687 0.702 0.695 0.584  
    31.2 0.401 0.424 0.413 0.302  
    15.6 0.282 0.291 0.287 0.176  
    7.8 0.182 0.175 0.179 0.068  
    0 0.113 0.108 0.111    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 85-96  
    1:2 Average % 89  
    Range % 84-95  
    1:4 Average % 93  
    Range % 89-97  
    1:8 Average % 90  
    Range % 85-94  
  • 回收率:
    The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 95-107  
    EDTA plasma (n=4) 92 88-100  
                 
                 
  • 研究领域:
    Cardiovascular
  • 引用文献:
    1. Procoagulant and Fibrinolytic Activity after Polytrauma in Rat , Wu X. et al,Am J Physiol Regul Integr Comp Physiol, 2015 Dec 2