试剂盒 > 酶联免疫试剂盒 > 大鼠免疫球蛋白G(IgG)ELISA Kit
    • ELISA Kit
    • 大鼠免疫球蛋白G(IgG)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    大鼠免疫球蛋白G(IgG)ELISA Kit

  • 英文名称:

    Rat Immunoglobulin G,IgG ELISA Kit

  • 货号:
    CSB-E07981r
  • 别名:
    N/A
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Rat
  • 其他种属:
  • 待测物名称:
    Immunoglobulin G,IgG
  • 缩写:
    IgG
  • 检测范围:
    31.25 ng/ml-8000 ng/ml
  • 灵敏度:
    31.25 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    8000 0.230 0.225 0.228    
    2000 0.417 0.407 0.412    
    500 0.643 0.664 0.654    
    125 1.238 1.257 1.248    
    31.25 1.754 1.770 1.762    
    0 2.035 1.988 2.012    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 100  
    Range % 95-104  
    1:2000 Average % 90  
    Range % 86-95  
    1:4000 Average % 87  
    Range % 83-92  
    1:8000 Average % 101  
    Range % 97-106  
  • 回收率:
    The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-95  
    EDTA plasma (n=4) 93 89-97  
                 
                 
  • 研究领域:
    Immunology