试剂盒 > 酶联免疫试剂盒 > 大鼠皮质醇(Cortisol)ELISA Kit
    • ELISA Kit
    • 大鼠皮质醇(Cortisol)ELISA Kit
    • 17,21-Dihydroxypregn-4-ene-3,20-dione. A 17-hydroxycorticosteroid with glucocorticoid and anti-inflammatory activities.From:MeSH[provided by RefSeq]
    • Pubchem440707
    • CAS no.152-58-9
    • Molecular FormulaC21H30O4
    • Molecular Weight346.4605
  • 产品类型:
    ELISA Kit
  • 产品名称:

    大鼠皮质醇(Cortisol)ELISA Kit

  • 英文名称:

    Rat Cortisol ELISA Kit

  • 货号:
    CSB-E05112r
  • 规格:
    96T
  • 中文价格:
    3900
  • 种属:
    Rat
  • 其他种属:
  • 待测物名称:
    Cortisol
  • 缩写:
    COR
  • 蛋白功能1:
    Metabolism
  • 检测范围:
    0.049 ng/ml-200 ng/ml
  • 灵敏度:
    0.049 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.195 0.185 0.190    
    50 0.336 0.321 0.329    
    12.5 0.515 0.553 0.534    
    3.12 0.913 0.982 0.948    
    0.78 1.334 1.261 1.298    
    0.195 1.663 1.656 1.660    
    0.049 1.933 1.900 1.917    
    0 2.326 2.402 2.364    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-98  
    1:2 Average % 96  
    Range % 92-104  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 90  
    Range % 83-97  
  • 回收率:
    The recovery of rat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-94  
    EDTA plasma (n=4) 96 92-100  
                 
                 
  • 研究领域:
    Metabolism
  • 引用文献:
    1. Stress Hormone and Reproductive System in Response to Honey Supplementation Combined with Different Jumping Exercise Intensities in Female Rats , Maryam Mosavat et al,BioMed Research International,2014;2014:123640
    2. Possible Activation of the Immune System by Chronic Peripheral Nesfatin-1 Application at the Acute Phase of Ischemia/Reperfusion Injury , ?mran Toru. et al,Erciyes Med J,2015,37(4):143-147