试剂盒 > 酶联免疫试剂盒 > 猪免疫球蛋白G(IgG)ELISA Kit
    • ELISA Kit
    • 猪免疫球蛋白G(IgG)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    猪免疫球蛋白G(IgG)ELISA Kit

  • 英文名称:

    Pig Immunoglobulin G(IgG) ELISA Kit

  • 货号:
    CSB-E06804p
  • 别名:
    N/A
  • 规格:
    96T
  • 中文价格:
    4200
  • 种属:
    Pig
  • 其他种属:
  • 待测物名称:
    Immunoglobulin G(IgG)
  • 缩写:
    IgG
  • 蛋白功能1:
    Immunoglobulin
  • 检测范围:
    0.586 μg/ml-150 μg/ml
  • 灵敏度:
    0.467 μg/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    150 0.254 0.251 0.253    
    37.5 0.555 0.517 0.536    
    9.38 0.755 0.721 0.738    
    2.34 0.999 0.981 0.990    
    0.58 1.169 1.113 1.141    
    0 1.481 1.465 1.473    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 90  
    Range % 85-96  
    1:400 Average % 95  
    Range % 91-107  
    1:800 Average % 93  
    Range % 86-99  
    1:1600 Average % 91  
    Range % 88-97  
  • 回收率:
    The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 88-98  
    EDTA plasma (n=4) 94 89-100  
                 
                 
  • 研究领域:
    Immunology
  • 引用文献:
    1. Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets , Fengrui Zhang et al,PLoS One,2013
    2. Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. , Wu S et al,Peptides,2012 Jun;35(2):225-30
    3. Oral administration of N-carbamylglutamate might improve growth performance and intestinal function of suckling piglets , Xiangfang Zeng.et al,Livestock Science,181: 242-248