试剂盒 > 酶联免疫试剂盒 > 小鼠丙二酸单酰辅酶A(malonyl CoA) ELISA kit
    • ELISA Kit
    • 小鼠丙二酸单酰辅酶A(malonyl CoA) ELISA kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    小鼠丙二酸单酰辅酶A(malonyl CoA) ELISA kit

  • 英文名称:

    Mouse malonyl coenzyme A ELISA kit

  • 货号:
    CSB-E12896m
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Mus musculus (Mouse)
  • 其他种属:
  • 待测物名称:
    malonyl coenzyme A
  • 缩写:
    malonyl CoA
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.214 2.298 2.256 2.159
    51.791 1.765 1.778 1.681
    2.51.224 1.268 1.246 1.149
    1.250.842 0.812 0.827 0.730
    0.6250.572 0.547 0.560 0.463
    0.3120.381 0.365 0.373 0.276
    0.1560.201 0.209 0.205 0.108
    00.096 0.098 0.097  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1000Average %90
    Range %84-996
    1:2000Average %95
    Range %89-99
    1:4000Average %99
    Range %95-105
    1:8000Average %98
    Range %90-106
  • 回收率:
    The recovery of mouse malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-96
    EDTA plasma (n=4)9587-99
  • 定性/定量:
    quantitative
  • 研究领域:
    Others
  • 引用文献:
    1. Fermentation Products of Commensal Bacteria Alter Enterocyte Lipid Metabolism , Ara煤jo JR,Cell Host Microbe,2020
    2. Malonylation of GAPDH is an inflammatory signal in macrophages , Galvan-Pena S, et al,Nature Communications,2019