试剂盒 > 酶联免疫试剂盒 > 小鼠诱导型一氧化氮合成酶(iNOS)ELISA Kit
    • ELISA Kit
    • 小鼠诱导型一氧化氮合成酶(iNOS)ELISA Kit
    • Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.[provided by RefSeq]
    • UniProtP29477
    • CUSABIOCSB-E08326m
  • 产品类型:
    ELISA Kit
  • 产品名称:

    小鼠诱导型一氧化氮合成酶(iNOS)ELISA Kit

  • 英文名称:

    Mouse inducible nitric oxide synthase,iNOS ELISA KIT

  • 货号:
    CSB-E08326m
  • 别名:
    Nos2 ELISA Kit; InoslNitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; Macrophage NOS ELISA Kit; MAC-NOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Mus musculus (Mouse)
  • 待测物名称:
    nitric oxide synthase 2, inducible
  • 缩写:
    NOS2
  • 检测范围:
    7.8 IU/mL-500 IU/mL
  • 灵敏度:
    1.95 IU/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    IU/ml.OD1OD2AverageCorrected
    5002.599 2.581 2.590 2.403
    2502.087 2.096 2.092 1.905
    1251.341 1.369 1.355 1.168
    62.50.760 0.798 0.779 0.592
    31.20.499 0.480 0.490 0.303
    15.60.344 0.368 0.356 0.169
    7.80.282 0.293 0.288 0.101
    00.186 0.188 0.187  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %96
    Range %90-102
    1:2Average %89
    Range %84-95
    1:4Average %102
    Range %98-107
    1:8Average %96
    Range %92-102
  • 回收率:
    The recovery of mouse iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9792-105
    EDTA plasma (n=4)9591-99
  • 定性/定量:
    quantitative
  • 研究领域:
    Neuroscience
  • 引用文献:
    1. 6-Gingerol abates benzo[a]pyrene-induced colonic injury via suppression of oxido-inflammatory stress responses in BALB/c mice , Ajayi BO, et al,Chemico-Biological Interactions,2019
    2. Directed self-assembly of herbal small molecules into sustained release hydrogels for treating neural inflammation , Zheng J, et al,Nature Communications,2019
    3. Pharmacological preconditioning with the cellular stress inducer thapsigargin protects against experimental sepsis , Wei Y, et al,Pharmacological research,2018
    4. HMBOX1 in hepatocytes attenuates LPS/D-GalN-induced liver injury by inhibiting macrophage infiltration and activation , HengliZhao.et al,Molecular Immunology,2018
    5. Development of Colorectal-Targeted Dietary Supplement Tablets Containing Natural Purple Rice Bran Oil as a Colorectal Chemopreventive , Busaban Sirithunyalug.et al,Nutrients,2018
    6. Multifunctional neuroprotective effect of Withanone, a compound from Withania somnifera roots in alleviating cognitive dysfunction , Pandey A.et al,Cytokine,2017
    7. Potassium 2-(l-hydroxypentyl)-benzoate attenuates neuroinflammatory responses and upregulates heme oxygenase-1 in systemic lipopolysaccharide-induced inflammation in mice , Chunyang Zhao.et al,Acta Pharmaceutica Sinica B,2017
    8. Hypericum Perforatum Extract inhibits Cigarette Smoke Induced Lung Inflammation , Halil Mahir Kaplan.et al,Science Signpost Publishing,2017
    9. Involvement of EGF receptor signaling and NLRP12 inflammasome in fine particulate matter-induced lung inflammation in mice. , Jin Y.et al,Environ Toxicol.,2016
    10. An analysis on the suppression of NO and PGE2 by diphenylheptane A and its effect on glycerophospholipids of lipopolysaccharide-induced RAW264.7 cells with UPLC/ESI-QTOF-MS. , She Y.et al,Anal Bioanal Chem.,2016