试剂盒 > 酶联免疫试剂盒 > 人载脂蛋白E(Apo-E)ELISA Kit
    • ELISA Kit
    • 人载脂蛋白E(Apo-E)ELISA Kit
    • Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.[provided by RefSeq]
    • UniProtP02649
    • BIOWWWAPOE
    • HGNC613
    • RGD2138
    • MGI88057
    • OMIM107741
    • CUSABIOCSB-E09748h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人载脂蛋白E(Apo-E)ELISA Kit

  • 英文名称:

    Human apolipoprotein E (Apo-E) ELISA Kit

  • 货号:
    CSB-E09748h
  • 别名:
    AD2, LDLCQ5, LPG, MGC1571, apolipoprotein E3
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    apolipoprotein E
  • 缩写:
    APOE
  • 蛋白功能1:
    Apolipoprotein
  • 蛋白功能3:
    Lipid transport
  • 检测范围:
    15.62 ng/ml-1000 ng/ml
  • 灵敏度:
    3.9 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    1000 0.088 0.090 0.089    
    500 0.135 0.142 0.139    
    250 0.227 0.237 0.232    
    125 0.324 0.341 0.333    
    62.5 0.583 0.598 0.591    
    31.25 0.847 0.864 0.856    
    15.62 1.228 1.235 1.232    
    0 2.155 2.199 2.177    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-95  
    1:200 Average % 102  
    Range % 97-107  
    1:400 Average % 91  
    Range % 85-97  
    1:800 Average % 97  
    Range % 91-103  
  • 回收率:
    The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 研究领域:
    Cardiovascular
  • 引用文献:
    1. Identification of Differentially Expressed Proteins in the Serum of Colorectal Cancer Patients Using 2D-DIGE Proteomics Analysis , Lay Cheng Lim,.et al,Pathology & Oncology Research,2016,22(1):169-177.