试剂盒 > 酶联免疫试剂盒 > 人载脂蛋白B100(Apo-B100)ELISA Kit
    • ELISA Kit
    • 人载脂蛋白B100(Apo-B100)ELISA Kit
    • This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.[provided by RefSeq]
    • UniProtP04114
    • BIOWWWAPOB
    • HGNC603
    • RGD2129
    • MGI88052
    • OMIM107730
    • CUSABIOCSB-E08100h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人载脂蛋白B100(Apo-B100)ELISA Kit

  • 英文名称:

    Human apolipoprotein B100 (Apo-B100) ELISA Kit

  • 货号:
    CSB-E08100h
  • 别名:
    FLDB, LDLCQ4, apoB-100|apoB-48|apolipoprotein B|apolipoprotein B48
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    apolipoprotein B (including Ag(x) antigen)
  • 缩写:
    APOB
  • 蛋白功能1:
    Apolipoprotein
  • 蛋白功能2:
    Lipogenesis and lipometabolism
  • 蛋白功能3:
    Cholesterol metabolism
  • 检测范围:
    78 ng/ml-5000 ng/ml
  • 灵敏度:
    19.5 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    5000 0.074 0.073 0.074    
    2500 0.118 0.121 0.120    
    1250 0.182 0.172 0.177    
    625 0.272 0.284 0.278    
    312 0.451 0.442 0.447    
    156 0.770 0.754 0.762    
    78 1.122 1.054 1.088    
    0 2.371 2.270 2.321    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 96  
    Range % 92-102  
    1:400 Average % 95  
    Range % 91-99  
    1:800 Average % 104  
    Range % 102-108  
    1:1600 Average % 87  
    Range % 81-92  
  • 回收率:
    The recovery of human Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 92-102  
    EDTA plasma (n=4) 90 87-94  
                 
                 
  • 研究领域:
    Cardiovascular
  • 引用文献:
    1. A study of serum Apo A-1 and Apo B-100 levels in women with preeclampsia , Timur H.et al,Pregnancy Hypertens.,6(2):121-5
    2. Prevention mechanism of 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside on lipid accumulation in steatosis hepatic L-02 cell , Wang W.et al,J Ethnopharmacol. ,2017 May 14;153(3):763-70.