试剂盒 > 酶联免疫试剂盒 > 人血管内皮细胞生长因子(VEGF)ELISA Kit
    • ELISA Kit
    • 人血管内皮细胞生长因子(VEGF)ELISA Kit
    • This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.[provided by RefSeq]
    • UniProtP15692
    • BIOWWWVEGFA
    • HGNC12680
    • RGD619991
    • MGI103178
    • OMIM192240
    • CUSABIOCSB-E11718h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人血管内皮细胞生长因子(VEGF)ELISA Kit

  • 英文名称:

    Human Vascular Endothelial cell Growth Factor(VEGF) ELISA KIT

  • 货号:
    CSB-E11718h
  • 别名:
    RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165|vascular permeability factor
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    vascular endothelial growth factor A
  • 缩写:
    VEGFA
  • 蛋白功能1:
    Cytokine
  • 蛋白功能3:
    Angiogenesis
  • 检测范围:
    31.25 pg/ml-2000 pg/ml
  • 灵敏度:
    25.297 pg/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.651 2.751 2.701 2.547
    10001.729 1.829 1.779 1.625
    5001.067 1.087 1.077 0.923
    2500.579 0.589 0.584 0.430
    1250.372 0.382 0.377 0.223
    62.50.255 0.265 0.260 0.106
    31.250.207 0.209 0.208 0.054
    00.153 0.154 0.154  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %98
    Range %94-106
    1:2Average %93
    Range %86-97
    1:4Average %87
    Range %82-93
    1:8Average %96
    Range %91-105
  • 回收率:
    The recovery of human VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9290-96
  • 研究领域:
    Cancer
  • 引用文献:
    1. Propranolol given orally for proliferating infantile haemangiomas: analysis of efficacy and serological changes in vascular endothelial growth factor and endothelial nitric oxide synthase in 35 patients , Yuan WL et al,Br J Oral Maxillofac Surg,2013 Jan 3. pii: S0266-4356(12)00623-7
    2. Preparation and characterization of decellularized tendon slices for tendon tissue engineering , /,journal of biomedical materials research part a,2012 Jun;100(6):1448-56.
    3. Expression and significance of vascular endothelial growth factor A and C in leukemia central nervous system metastasis , Tang YT et al,Leuk Res,2013 Apr;37(4):359-66.
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