试剂盒 > 酶联免疫试剂盒 > 人催乳素(PRL/LTH) ELISA Kit
    • ELISA Kit
    • 人催乳素(PRL/LTH) ELISA Kit
    • A segmental duplication of the p arm of chromosome 16 created two identical copies of the GIY-YIG domain containing gene, this record represents the more centromeric copy. Exons of this gene overlap with exons of the phenol-preferring sulfotransferase (SULT1A3) gene. Two transcript variants that encode different protein isoforms have been identified through sequence analysis.[provided by RefSeq]
    • UniProtP01236
    • BIOWWWPRL
    • HGNC9089
    • RGD621047
    • MGI1858197
    • OMIM605961
    • CUSABIOCSB-E06883h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人催乳素(PRL/LTH) ELISA Kit

  • 英文名称:

    Human Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 货号:
    CSB-E06883h
  • 别名:
    N/A
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Homo sapiens (Human)
  • 其他种属:
  • 待测物名称:
    prolactin
  • 缩写:
    PRL
  • 蛋白功能1:
    Sex hormone
  • 蛋白功能3:
    Lactation
  • 检测范围:
    40 μIU/mL-2000 μIU/mL
  • 灵敏度:
    50 μIU/mL
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average Corrected  
    2000 2.186 2.105 2.146 2.115  
    800 1.201 1.197 1.199 1.168  
    300 0.622 0.619 0.621 0.590  
    100 0.296 0.302 0.299 0.268  
    40 0.145 0.148 0.147 0.116  
    0 0.032 0.030 0.031    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 86-97  
    1:2 Average % 92  
    Range % 87-100  
    1:4 Average % 95  
    Range % 92-99  
    1:8 Average % 95  
    Range % 90-98  
  • 回收率:
    The recovery of human PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-102  
    EDTA plasma (n=4) 97 92-99  
                 
                 
  • 定性/定量:
    quantitative
  • 研究领域:
    Signal Transduction
  • 引用文献:
    1. In Vivo Evidence for Epidermal Growth Factor Receptor (EGFR)-mediated Release of Prolactin from the Pituitary Gland , Maik Dahlhoff et al,The Journal of Biological Chemistry,2011 Nov 11;286(45):39297-306.
    2. YAP mediates human decidualization of the uterine endometrial stromal cells , Hengxi Chen.et al,Placenta,May 2017