试剂盒 > 酶联免疫试剂盒 > 人骨桥素(OPN)ELISA Kit
    • ELISA Kit
    • 人骨桥素(OPN)ELISA Kit
    • no[provided by RefSeq]
    • UniProtP10451
    • BIOWWWSPP1
    • HGNC24343
    • RGD1310755
    • MGI1921572
    • OMIM609150
    • CUSABIOCSB-E08392h
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人骨桥素(OPN)ELISA Kit

  • 英文名称:

    Human Osteopontin,OPN ELISA Kit

  • 货号:
    CSB-E08392h
  • 别名:
    BNSP, BSPI, ETA-1, MGC110940, OPN, SPP1/CALPHA1 fusion|bone sialoprotein I|early T-lymphocyte activation 1|osteopontin|osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein|s
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    secreted phosphoprotein 1
  • 缩写:
    OPN
  • 蛋白功能1:
    Tumor marker
  • 蛋白功能3:
    Biomineralization
  • 检测范围:
    0.78 ng/ml-50 ng/ml
  • 灵敏度:
    0.195 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.418 2.333 2.376 2.271
    251.684 1.706 1.695 1.590
    12.51.175 1.152 1.164 1.059
    6.250.654 0.664 0.659 0.554
    3.120.383 0.361 0.372 0.267
    1.560.245 0.232 0.239 0.134
    0.780.176 0.185 0.181 0.076
    00.102 0.108 0.105  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:5Average %94
    Range %88-98
    1:10Average %100
    Range %94-104
    1:20Average %93
    Range %88-97
    1:40Average %99
    Range %93-104
  • 回收率:
    The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9581-97
    EDTA plasma (n=4)10297-106
  • 研究领域:
    Cancer
  • 引用文献:
    1. Combination Treatment with Whole Body Vibration and a Kidney-tonifying Herbal Fufang Prevent Osteoporosis in Ovariectomized Rats , Wei QS et al,Orthop Surg,2017 Feb;7(1):57-65.
    2. Pathogenesis of Multiple Lentigines in LEOPARD Syndrome with PTPN11 Gene Mutation. , Motegi SI. et al,Acta Derm Venereol.,2015 Nov 4;95(8):978-84.
    3. Serum osteopontin levels in relation to bone mineral density and bone turnover markers in postmenopausal women , Wei QS. et al,Scand J Clin Lab Invest,2015 Sep 28:1-7.
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